REU/RUI: Cytogenetic and Microspectrophotometric Investigations of Multinucleate Marine Green Algae

REU/RUI：多核海洋绿藻的细胞遗传学和显微分光光度研究

• 批准号: 8817846
• 负责人: Donald Kapraun
• 金额: $ 9.77万
• 依托单位: University of North Carolina at Wilmington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-01-15 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8817846&HistoricalAwards=false
• 关键词: REU; RUI; Cytogenetic; Microspectrophotometric; Investigations

Working with talented undergraduates, Dr. Donald Kapraun of the University of North Carolina at Wilmington will study several groups of marine green algae, using modern methods of microscopy including microspectrophotometric measures of DNA content. The work focuses on basic patterns of chromosomal change that accompany speciation in three major groups of multinucleate green algae. Information will be obtained concerning: basic chromosome number; symmetry of chromosome complements; euchromatin and heterochromatin content; nuclear DNA quantity; and guanosine- cytosine content in nuclear DNA. The objective of the research is to determine what chromosomal modifications correlate with species diversity in the three groups of marine green algae. In conjunction with morphological features and other evidence, such data should prove useful in reconstructing phylogenetic relationships among these plants. In addition, the study will determine whether marine multinucleate forms, including cellular, septate coenocytes and non-septate coenocytes, manifest particular and unique types of chromosomal transformations.

北卡罗来纳大学威尔明顿分校的 Donald Kapraun 博士将与才华横溢的本科生合作，利用现代显微镜方法（包括 DNA 含量的显微分光光度测量方法）研究几类海洋绿藻。 这项工作重点关注三大多核绿藻类群中伴随物种形成的染色体变化的基本模式。 将获得以下信息： 基本染色体数目；染色体互补体的对称性；常染色质和异染色质含量；核DNA数量；以及核DNA中的鸟苷-胞嘧啶含量。 该研究的目的是确定哪些染色体修饰与三类海洋绿藻的物种多样性相关。 结合形态特征和其他证据，这些数据应该有助于重建这些植物之间的系统发育关系。 此外，该研究还将确定海洋多核形式，包括细胞、有隔膜的双核细胞和无隔膜的双核细胞，是否表现出特殊和独特类型的染色体转化。