The Role of G4-DNA in the Pairing of Chromosomes

G-4 DNA是一个四链结构，包括四个平行的，相同的，富含鸟嘌呤的序列，鸟嘌呤hoogsteen配对。这样的元素能够交联四个沃森-克里克双链。由于在减数分裂期间，DNA复制后的染色体配对涉及每个染色单体的四个拷贝，它们必须在重组发生之前连接在一起，因此我们认为DNA中富含鸟嘌呤的基序的自我识别形成G4-DNA交联导致突触。G4-DNA交联也可用于有丝分裂和多丝染色体形成过程中的染色体排列。为了验证G4-DNA形成区作为染色体配对的识别信号的假设，我们将使用抗G4-DNA抗体在减数分裂配对过程中检测染色体中的G4-DNA。将开发抗g4 - dna抗体，并使用过氧化物酶和荧光方法检测可能交联染色体的G-4区域。这将需要广泛使用高分辨率显微镜来检查减数分裂细胞中的染色体。这项工作的结果，如果支持工作假设，将导致我们对基因遗传的一个基本方面的理解的重大突破，减数分裂分离。

G-4 DNA is a four-stranded structure involving four parallel, identical, guanine-rich sequences, with the guanines Hoogsteen-paired. Such elements are able to crosslink four Watson-Crick duplexes. Since during meiosis the pairing of chromosomes after DNA replication involves four copies of each chromatid, which must synapse together before recombination takes place, we suggest that a self-recognition of guanine-rich motifs in DNA to form G4-DNA crosslinks cause the synapsis. G4-DNA crosslinks may also be used to align chromosomes in mitosis and in the formation of polytene chromosomes. To test the hypothesis that G4-DNA-forming regions serve as identification signals for chromosome matching, anti-G4-DNA antibodies will be used to detect G4- DNA in chromosomes during meiotic pairing. Anti-G4-DNA antibodies will be developed and used to detect G-4 regions that may crosslink chromosomes both with peroxidase and fluorescence methods. This will require extensive use of high-resolution microscopy to examine chromosome figures in meiotic cells. The results of this work, if supportive of the working hypothesis, will result in a major breakthrough in our understanding of a fundamental aspect of genetic inheritance, meiotic segregation.