Support of the LSU Collection of Frozen Tissues

路易斯安那州立大学冷冻组织收藏的支持

• 批准号: 8818319
• 负责人: Herbert Dessauer
• 金额: $ 15.94万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-10-15 至 1995-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8818319&HistoricalAwards=false
• 关键词: Support; LSU; Collection; Frozen; Tissues

Many recent advances in biological sciences have resulted from biochemical studies focused at the molecular level. The source of these molecules is frozen tissue. The Frozen Tissue Collection at the Louisiana State University Museum of Natural Science is the largest of its kind. It contains tissue samples of over 30,000 vertebrate animals, each preserved at -70 C. Ultracold preservation insures that proteins and the hereditary information itself, DNA, remain in a physiologically active (viable) state. The collection is used for studies of comparative biochemistry, physiology, genetics, forensics and evolution. Researchers throughout the United States and Canada regularly use the collection, which clearly constitutes a national resource, one not replicated in the U.S. or elsewhere. Frozen tissue collections are costly to maintain and operate. Expensive freezers are required for ultracold preservation. Manipulation of samples is very labor intensive, because each specimen must remain frozen (specimens must be sorted on dry ice). LSU currently supplies a Ph.D. level curator, a graduate curatorial assistant (50% time), and utility costs for freezers. This project provides funds for needed equipment and labor to manage and computerize the collection and make it a fully functional national resource, one capable of accepting and dispersing tissue samples to the research community. The project will ensure that these irreplaceable research materials are well maintained and made readily available to the research community.

生物科学的许多最新进展都是集中在分子水平的生化研究的结果。 这些分子的来源是冷冻组织。 路易斯安那州立大学自然科学博物馆的冷冻组织收藏是同类中最大的。 它包含 30,000 多个脊椎动物的组织样本，每个样本均保存在 -70°C 下。超冷保存可确保蛋白质和遗传信息本身、DNA 保持生理活性（活力）状态。 该藏品用于比较生物化学、生理学、遗传学、法医学和进化论的研究。 美国和加拿大各地的研究人员经常使用该馆藏，这显然构成了一种国家资源，在美国或其他地方都没有复制。 冷冻组织收集的维护和操作成本高昂。 超冷保存需要昂贵的冰箱。 样品的操作是非常劳动密集型的，因为每个标本都必须保持冷冻状态（标本必须在干冰上分类）。 LSU 目前提供博士学位。一级策展人、一名研究生策展助理（50％的时间）以及冰箱的公用事业成本。 该项目为管理和计算机化收集所需的设备和劳动力提供资金，使其成为功能齐全的国家资源，能够接受组织样本并将其分发给研究界。 该项目将确保这些不可替代的研究材料得到良好维护并可供研究界随时使用。