Salt Bridges in a-Helices Formed by Short Peptides of de Novo Design

由 de Novo 设计的短肽形成的 a 螺旋中的盐桥

• 批准号: 8818398
• 负责人: Robert Baldwin
• 金额: $ 32.8万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-01-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8818398&HistoricalAwards=false
• 关键词: Salt; Bridges; Helices; Formed; Short

Dr. Baldwin will continue his investigation of the stabilization forces of short helical peptides. He has previously shown that short peptides are able to form monomeric -helices in aqueous solution. Helical stability depended strongly on residue spacing in this set of peptides and therefore helix stabilization by salt bridges was likely. He will now extend this study to include other pairs of charged residues potentially able to form salt bridges in order to evaluate the helix-stabilizing properties of these salt bridges, if they exist, and to investigate further the mechanism of -helix stabilization by salt bridges and non-H- bonded ion pairs. Salt bridges formed by short helices of de novo design are being studied by only a few scientists. The study of designed helices is closely related to the study of -helix formation by naturally occurring peptides derived from helix- containing segments of peoteins. The broad significance of this work is that short helices, formed at early stages in the folding process, have the ability to play a directing role in the pathway of protein folding.***//

鲍德温博士将继续调查 短螺旋肽的稳定力。 他 先前表明短肽能够形成 单体-水溶液中的螺旋。 螺旋稳定性 强烈依赖于这组肽中的残基间隔 因此可能通过盐桥来稳定螺旋。 他现在将扩展这项研究，包括其他对 带电残基可能形成盐桥， 为了评估这些材料的螺旋稳定特性， 盐桥，如果存在的话，并进一步调查 盐桥和非H- 键合离子对 由从头设计的短螺旋形成的盐桥是 只有少数科学家在研究。 研究 螺旋的设计与螺旋的研究密切相关 由螺旋衍生的天然存在的肽形成- 含有甜菜碱的片段。 的广泛意义 这项工作是短螺旋，形成于早期阶段， 折叠过程中，有能力发挥指导作用 在蛋白质折叠途径中的作用 ***//