H-Exchange Rates and Hydrogen Bonding of Individual Peptide NH Protons in Alpha-Helices
α 螺旋中单个肽 NH 质子的 H 交换率和氢键
基本信息
- 批准号:9208648
- 负责人:
- 金额:$ 26.4万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:1992
- 资助国家:美国
- 起止时间:1992-08-01 至 1996-01-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Dr. Baldwin proposes to measure, for the first time, exchange rates of individual peptide NH protons within single, isolated alpha- helices. A nuclear magnetic resonance (NMR) technique will be used, based on 15N spectral editing of the one-dimensional proton spectrum. A single peptide NH will be 15N-labeled by chemical synthesis of the peptide, and 15N spin echo difference spectroscopy will be used to give the 1H spectrum of the single 15N-labeled peptide NH. A trial experiment demonstrating the feasibility of this approach has been made. The new method will be used to test the basic assumptions of helix-random coil transition theory, and also to provide background information about exchange rates of amide protons in single alpha-helices, for use in interpreting data on protein folding intermediates. %%% The proposed experiments will provide a new technique for obtaining structural information about short peptides, for example, when the peptide is complexed with a specific receptor. Currently there is wide interest in " peptide libraries", both in the biotechnology field and in biomedical science generally. Methodologies have been developed for synthesizing astronomical numbers of different short peptide sequences and then for selecting a small number of sequences that can bind specifically to a receptor in a signal transduction pathway. This method will provide an important tool for characterizing the structure of a peptide in a peptide-receptor complex. As pointed out above, the method also has direct application to the problem of characterizing structures of kinetic intermediates on the pathway of protein folding.
鲍德温博士首次提出衡量汇率 单个肽NH质子在单个分离的α- 螺旋 核磁共振(NMR)技术将被 使用,基于一维质子的15 N光谱编辑 江西篇章 单个肽NH将通过化学方法被15 N标记。 肽的合成和15 N自旋回波差谱 将用于给出单个15 N标记的1H光谱 肽NH。 一个试验性的实验证明了 已经采取了这种做法。 新方法将用于测试 螺旋-无规卷曲转变理论的基本假设,以及 还提供了有关汇率的背景资料, 单α-螺旋中的酰胺质子,用于解释数据 蛋白质折叠中间体。 %%% 这些实验将为获得 关于短肽的结构信息,例如,当 肽与特定受体复合。 目前还 广泛的兴趣“肽库”,无论是在生物技术 在生物医学领域,一般来说。 方法论一直是 用来合成不同短的天文数字 肽序列,然后选择少量的 可以特异性结合信号受体的序列 转导途径 这种方法将提供一种重要的工具 用于表征肽受体中肽的结构 复杂. 如上所述,该方法还具有直接 应用于表征动力学结构的问题 蛋白质折叠途径的中间体。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Robert Baldwin其他文献
Globi Neerlandici: The Production of Globes in the Low Countries@@@Globes from the Western World
Globi Neerlandici:低地国家的地球仪@@@来自西方世界的地球仪
- DOI:
- 发表时间:
1996 - 期刊:
- 影响因子:0
- 作者:
Robert Baldwin;P. V. D. Krogt;Elly Dekker - 通讯作者:
Elly Dekker
Accuracy of CT biopsy: laser guidance versus conventional freehand techniques.
CT 活检的准确性:激光引导与传统徒手技术。
- DOI:
- 发表时间:
1998 - 期刊:
- 影响因子:4.8
- 作者:
F. S. Pereles;M. Baker;Robert Baldwin;E. Krupinski;E. Unger - 通讯作者:
E. Unger
Cognitive ability and creativity: Typology contributions and a meta-analytic review
- DOI:
10.1016/j.intell.2023.101757 - 发表时间:
2023-05-01 - 期刊:
- 影响因子:
- 作者:
Andra Serban;Sven Kepes;Wenhao Wang;Robert Baldwin - 通讯作者:
Robert Baldwin
Where Risk Society Meets the Regulatory State: Exploring Variations in Risk Regulation Regimes
- DOI:
10.1057/palgrave.rm.8240012 - 发表时间:
1999-01-01 - 期刊:
- 影响因子:1.700
- 作者:
Christopher Hood;Henry Rothstein;Robert Baldwin;Judith Rees;Michael Spackman - 通讯作者:
Michael Spackman
2D to 3D body pose estimation for sign language with Deep Learning
通过深度学习对手语进行 2D 到 3D 身体姿势估计
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
Jacob Murray;Robert Baldwin;Donald L. Hagan;Patrick Hiesl - 通讯作者:
Patrick Hiesl
Robert Baldwin的其他文献
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{{ truncateString('Robert Baldwin', 18)}}的其他基金
Planning Grant to Establish an Alternate Site for MAST at the Colorado School of Mines
规划拨款在科罗拉多矿业学院建立 MAST 备用地点
- 批准号:
0000405 - 财政年份:2000
- 资助金额:
$ 26.4万 - 项目类别:
Standard Grant
Conference on Research in Income and Wealth to be held in Washington D.C., June 1995: Geography and Ownership as Bases for Economic Accounting
收入和财富研究会议将于 1995 年 6 月在华盛顿特区举行:地理和所有权作为经济核算的基础
- 批准号:
9409757 - 财政年份:1994
- 资助金额:
$ 26.4万 - 项目类别:
Standard Grant
Salt Bridges in a-Helices Formed by Short Peptides of de Novo Design
由 de Novo 设计的短肽形成的 a 螺旋中的盐桥
- 批准号:
8818398 - 财政年份:1989
- 资助金额:
$ 26.4万 - 项目类别:
Continuing Grant
Extraction of Minerals Using Supercritical Fluid Technology
使用超临界流体技术提取矿物
- 批准号:
8714509 - 财政年份:1987
- 资助金额:
$ 26.4万 - 项目类别:
Standard Grant
Engineering Research Equipment Grants: Fourier Transform Infrared Spectrophotometer
工程研究设备补助金:傅里叶变换红外分光光度计
- 批准号:
8705138 - 财政年份:1987
- 资助金额:
$ 26.4万 - 项目类别:
Standard Grant
Relation Between the Protein Stability of Ribonuclease S andthe Stability of the Isolated S-peptide Helix
核糖核酸酶S蛋白稳定性与分离S肽螺旋稳定性的关系
- 批准号:
8518112 - 财政年份:1986
- 资助金额:
$ 26.4万 - 项目类别:
Continuing Grant
The Recognition Complex Formed By S-Peptide and S-Protein During Folding
S肽和S蛋白折叠过程中形成的识别复合物
- 批准号:
8209296 - 财政年份:1982
- 资助金额:
$ 26.4万 - 项目类别:
Continuing Grant
Tertiary Structure of Dna in Phage Lambda
噬菌体 Lambda 中 DNA 的三级结构
- 批准号:
8022706 - 财政年份:1981
- 资助金额:
$ 26.4万 - 项目类别:
Continuing Grant
U.S. Policies in Response to Growing International Trade Competitiveness
美国应对日益增长的国际贸易竞争力的政策
- 批准号:
8116448 - 财政年份:1981
- 资助金额:
$ 26.4万 - 项目类别:
Standard Grant
Specialized Research Equipment Grant: Gas Chromatograph For Research in Coal Hydrogenation Kinetics
专业研究设备补助金:用于煤加氢动力学研究的气相色谱仪
- 批准号:
7925764 - 财政年份:1980
- 资助金额:
$ 26.4万 - 项目类别:
Standard Grant
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