Isolation and Characterization of Cytokinin Metabolic Enzymes

细胞因子是一类在植物生长发育中起重要作用的激素 在细胞分裂和分化中起作用。 控制机制 对植物中细胞分裂素的适当水平知之甚少。 该研究项目的长期目标是确定 控制细胞分裂素代谢的调控元件 菜豆最近的研究发现了基因差异 在菜豆胚细胞分裂素代谢中， 新的玉米素代谢物和三种细胞分裂素特异性的分离 内切酶由于这些酶的活性是物种特异性的， 产生的代谢产物显示出比 在母体化合物中，关键代谢酶的选择性表达可 是调节活性细胞分裂素水平的重要机制。 为了阐明代谢控制机制，我们建议 为了进一步表征酶， 用于检测不同发育阶段酶活性的抗体 阶段，并进行分子分析的调节， 这些酶的表达。 拟议的工作对 制定一个工作模式， 高等植物激素代谢 细胞分裂素是一类调节植物生长的激素 和细胞分裂。 提案工作旨在隔离 并确定这些植物激素及其功能的各个方面 更好地了解植物生长。

Cytokin are a group of plant hormones which play an important role in cell division and differentiation. The mechanisms controlling the appropriate levels of cytokinins in plants are poorly understood. The long term goal of the research project is to identify regulatory elements controlling cytokinin metabolism in the genus Phaseolus. Recent findings led to the discovery of genetic differences in cytokinin metabolism in Phaseolus embryos, identification of novel zeatin metabolites and isolation of three cytokinin specific enzymes. As the activities of these enzymes are species specific and the resulting metabolites display higher biological activity than the parent compound, selective expression of key metabolic enzymes may be a significant mechanism of regulating active cytokinin levels. To elucidate the metabolic control mechanisms, we propose to further characterize the enzymes, to generate mononclonal anti bodies for detection of enzyme activity at different developmental stages and to conduct molecular analyses of the regulation and expression of these enzymes. The proposed work is important to the formulation of a working model governing the overall controls of hormone metabolism in higher plants. Cytokinins are a group of hormones which regulate plant growth and cell division. The proposal work is designed to isolate and identify aspects of these plant hormones and their functioning to better understand plant growth.