Properties of Carbonic Anhydrase in Chlamydomonas reinhardtii

莱茵衣藻碳酸酐酶的性质

• 批准号: 8818845
• 负责人: H. David Husic
• 金额: $ 7.81万
• 依托单位: Lafayette College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-06-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8818845&HistoricalAwards=false
• 关键词: Properties; Carbonic; Anhydrase; Chlamydomonas; reinhardtii

Carbonic anhydrase is an enzyme which catalyzes the interconversion of the two forms of inorganic carbon, carbon dioxide and bicarbonate ion, which predominate under physiological conditions. In various species of unicellular green algae, including Chlamydomonas reinhardtii, one or more forms of carbonic anhydrase are critical components of an inorganic carbon concentrating system which allows these organisms to efficiently utilize the low levels of ambient carbon dioxide which would otherwise limit their growth. This project investigates the nature of the structure of the extracellular form of carbonic anhydrase in Chlamydomonas reinhardtii in vivo an in vitro and examines the effect of this structure on the catalytic properties of the enzyme. The localization of the extracellular enzyme in C. reinhardtii will be more clearly defined by chemical cross- linking and direct cellular binding studies. In addition, the stabilization, purification and localization of the intracellular form(s) of the enzyme will be investigated. These studies are critical in terms of identifying the components, and describing the details of the mechanism by which Chlamydomonas reinhardtii is able to efficiently concentrate inorganic carbon to provide carbon dioxide for photosynthesis. It is potentially useful for improving photosynthetic capacity of crop plants.***//

碳酸酐酶是一种催化无机碳、二氧化碳和碳酸氢根离子两种形式相互转化的酶，这两种形式在生理条件下占主导地位。 在各种单细胞绿藻中，包括莱茵衣藻，一种或多种形式的碳酸酐酶是无机碳浓缩系统的关键组成部分，该系统使这些生物体能够有效地利用低水平的环境二氧化碳，否则会限制其生长。 该项目在体内和体外研究莱茵衣藻细胞外形式碳酸酐酶的结构性质，并检查该结构对酶催化特性的影响。 化学交联和直接细胞结合研究将更清楚地确定莱茵衣藻细胞外酶的定位。 此外，还将研究酶的细胞内形式的稳定性、纯化和定位。 这些研究对于确定成分和描述莱茵衣藻能够有效浓缩无机碳以提供用于光合作用的二氧化碳的机制细节至关重要。 它对于提高农作物的光合作用能力具有潜在用途。***//