Properties of Carbonic Anhydrase in Chlamydomonas Reinhardtii

莱茵衣藻碳酸酐酶的性质

• 批准号: 9117563
• 负责人: H. David Husic
• 金额: $ 9.6万
• 依托单位: Lafayette College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-01 至 1995-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9117563&HistoricalAwards=false
• 关键词: Properties; Carbonic; Anhydrase; Chlamydomonas; Reinhardtii

The enzyme carbonic anhydrase catalyzes the interconversion of the two forms of inorganic carbon, CO2 and HCO3, which predominate in aqueous media at physiological pH values. In the alga Chlamydomonas reinhardtii, one or more forms of carbonic anhydrase are components of a system for inorganic carbon acquisition and concentration. This system enables these organisms to efficiently utilize CO2 present at low levels, which could otherwise limit their growth. A carbonic anhydrase-specific active site-directed radiolabeled photoaffinity reagent will be synthesized in order to study the physical relationship between the large and small subunits with respect to the active site of the enzyme, and as a tool in the identification and isolation of physiologically important intracellular forms of carbonic anhydrase which are present in levels too low to be readily identified and quantified by currently available methods. This proposal will investigate the relationship between the subunit structure and disulfide bond reduction status on both the catalytic and inhibitor binding properties of the predominant extracellular form of carbonic anhydrase from Chlamydomonas reinhardtii. These studies will contribute to understanding the structure-function relationship of the extracellular carbonic anhydrase, and to the identification of other important components of the system by which Chlamydomonas reinhardtii cells are able to efficiently concentrate inorganic carbon to provide CO2 for photosynthesis.

碳酸酐酶催化 无机碳的两种形式，CO2和HCO3， 在生理pH值下主要存在于水性介质中。 在 莱茵衣藻，一种或多种 碳酸酐酶是无机酶系统的组成部分， 碳收集和浓缩。该系统使这些 生物体有效利用低水平的CO2， 否则会限制它们的生长。 碳酸 脱水酶特异性活性定点放射性标记 合成光亲和试剂，以研究 大小亚基之间的物理关系， 关于酶的活性位点，作为一种工具， 鉴定和分离生理上重要 碳酸酐酶的细胞内形式，其存在于 水平太低，无法通过 目前可用的方法。 该提案将调查 亚基结构与二硫键的关系 催化剂和抑制剂上的键还原状态 主要细胞外形式的结合特性 来自莱茵衣藻的碳酸酐酶。 这些 研究将有助于理解结构-功能 细胞外碳酸酐酶的关系，以及 识别系统的其他重要组成部分 莱茵衣藻细胞能够通过这种方式 有效地浓缩无机碳， 光合作用