Biochemical Genetics and Gibberellin Biosynthesis in Maize

玉米的生化遗传学和赤霉素生物合成

• 批准号: 8819809
• 负责人: Bernard Phinney
• 金额: $ 49.08万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8819809&HistoricalAwards=false
• 关键词: Biochemical; Genetics; Gibberellin; Biosynthesis; Maize

This research analyzes gibberellin (GA) biosynthesis in relation to shoot growth in maize. The approach combines the disciplines of chemistry (organic synthesis), biochemistry (radiolabelled feeds and enzymology) and genetics (molecular and physiological). The specific goals of this research are to use GA mutants (and normals): (1) to analyze gene expression in callus tissue cultures; (2) to identify maize GA conjugates and analyze conjugation in seedlings; (3) to correlate levels of GA1 with the short and intermediate alleles of d1 mutant seedlings; (4) to locate the steps in the GA biosynthetic pathway blocked by the d2, d3 and an1 mutants; (5) to analyze the biosynthetic origin of GA3 in maize shoots from feeding studies with labelled GA4, GA7 and 2,3-dehydro-GA9; (6) to study the properties of normal and mutant hydroxylase, the enzyme controlling the step GA20 to GA1; (7) to continue preliminary studies on cloning the dwarfing genes; and (8) to continue classical genetic studies with mutants of maize concerned with GA metabolism. GAs are plant hormones that regulate many plant responses including shoot elongation. In maize, plants lacking endogenous GA1 possess a dwarf habit of growth. Five dwarf mutants (GA mutants) are used to unravel details of the GA biosynthetic pathway, and to define the role of specific GAs in the control of GA dependent shoot elongation.

本研究分析了赤霉素（GA）生物合成与 to shoot射击growth生长in maize玉米. 该方法结合了学科 化学（有机合成）、生物化学（放射性标记 饲料和酶学）和遗传学（分子和生理学）。 这项研究的具体目标是使用GA突变体（和 （1）分析愈伤组织中的基因表达 （2）鉴定玉米GA缀合物并分析 （3）将GA 1水平与植物生长因子之间的相关性进行了研究。 d1突变体幼苗的短等位基因和中间等位基因; 以定位GA生物合成途径中被 d_2、d_3和an_1突变体;（5）分析其生物合成来源 从用标记的GA 4进行的饲养研究中， GA 7和2，3-脱氢-GA 9;（6）研究了正常 和突变羟化酶，所述酶控制步骤GA 20， GA 1;（7）继续进行矮生无性系克隆的初步研究 基因;和（8）继续经典的遗传学研究， 与GA代谢有关的玉米突变体。 GA是调节许多植物反应的植物激素 包括芽伸长。 在玉米中，缺乏内源性 GA 1具有矮化生长的习性。 5个矮秆突变体（GA 突变体）用于解开GA生物合成的细节 途径，并确定特定的GAs在控制 GA依赖的芽伸长。