Gibberellin Biosynthesis in Maize-Chemistry, Biochemistry and Genetics

9604460 Phinney这个实验室的总体研究目标是了解依赖赤霉素(GA)的玉米新梢生长的生化、遗传和分子基础。该计划对科学进步很重要，因为结果确定了玉米生长的控制因素，玉米是社会福利的重要经济作物。赤霉素是一种植物激素，是新梢生长的控制器。它们存在于整个植物界。首席调查员(PI)率先使用单基因突变来揭示GA生物合成途径的细节；这些突变还被用于评估特定赤霉素在其生长控制中的生物学意义。计划中的计划将继续对玉米赤霉素的研究，这将(I)确定特定玉米赤霉素的生物合成来源和代谢，(Ii)定位被矮化突变体(GA-突变体)阻断的途径中的特定步骤，以及(Iii)筛选转座子标记的GA-矮化突变体，目的是克隆和表达控制玉米生物合成途径步骤的基因。该项目致力于赤霉素的生物合成途径，赤霉素是一种调节枝条生长的植物激素。将使用化学和遗传实验相结合的方法来研究玉米中赤霉素的产生方式。这项工作很重要，因为它将使人们更好地理解植物生长是如何控制的。这项工作具有基本的和实际的重要性，并可能最终导致作物产量的提高和世界粮食供应的增加。

9604460 Phinney The overall research goal of this laboratory is to understand the biochemical, genetic and molecular basis for gibberellin (GA)-dependent shoot growth in maize. The program is important to the advancement of science in that the results define control elements for the growth of maize, an important economic plant for the welfare of society. Gibberellins are plant hormones that are controllers of shoot growth. They are present throughout the plant kingdom. The Principal Investigator (PI) has pioneered the use of single gene mutants to unravel the details of the GA-biosynthetic pathway; these mutants have also been used to evaluate the biological significance of specific gibberellins in their control of growth. The projected program will continue studies on the gibberellins of maize which will (i) define the biosynthetic origin and metabolism of specific maize gibberellins, (ii) locate specific steps in the pathway that are blocked by dwarf mutants (GA-mutants) and (iii) screen for transposon tagged GA-dwarf mutants with a goal of cloning and expressing the genes that control steps in the maize biosynthetic pathway. This project addresses the biological routes for synthesis of gibberellins, plant hormones that regulate shoot growth. The way gibberellins are made in corn will be studied using combination of chemical and genetic experimental approaches. The work is important because it will lead to a greater understanding of how plant growth is controlled. This work has both basic and practical importance and may eventually lead to increased crop productivity and an increase in the world's food supplies.