Gibberellin Biosynthesis in Maize-Chemistry, Biochemistry and Genetics

9604460 Phinney该实验室的总体研究目标是了解玉米中赤霉素（GA）依赖的芽生长的生物化学，遗传和分子基础。该计划对科学的进步很重要，因为其结果确定了玉米生长的控制因素，玉米是一种重要的经济作物，有利于社会福利。赤霉素是植物激素，是芽生长的控制器。它们存在于整个植物王国。主要研究者（PI）率先使用单基因突变体来解开GA生物合成途径的细节;这些突变体也被用来评估特定赤霉素在其生长控制中的生物学意义。该计划将继续研究玉米赤霉素，将（i）确定特定玉米赤霉素的生物合成起源和代谢，（ii）定位被矮化突变体（GA突变体）阻断的途径中的特定步骤，以及（iii）筛选转座子标记的GA矮化突变体，目标是克隆和表达控制玉米生物合成途径中步骤的基因。 该项目研究赤霉素的生物合成途径，赤霉素是调节植物生长的激素。将采用化学和遗传实验方法相结合的方式研究赤霉素在玉米中的产生。这项工作很重要，因为它将导致更好地了解植物生长是如何控制的。这项工作具有基本和实际的重要性，并可能最终导致作物产量的提高和世界粮食供应的增加。

9604460 Phinney The overall research goal of this laboratory is to understand the biochemical, genetic and molecular basis for gibberellin (GA)-dependent shoot growth in maize. The program is important to the advancement of science in that the results define control elements for the growth of maize, an important economic plant for the welfare of society. Gibberellins are plant hormones that are controllers of shoot growth. They are present throughout the plant kingdom. The Principal Investigator (PI) has pioneered the use of single gene mutants to unravel the details of the GA-biosynthetic pathway; these mutants have also been used to evaluate the biological significance of specific gibberellins in their control of growth. The projected program will continue studies on the gibberellins of maize which will (i) define the biosynthetic origin and metabolism of specific maize gibberellins, (ii) locate specific steps in the pathway that are blocked by dwarf mutants (GA-mutants) and (iii) screen for transposon tagged GA-dwarf mutants with a goal of cloning and expressing the genes that control steps in the maize biosynthetic pathway. This project addresses the biological routes for synthesis of gibberellins, plant hormones that regulate shoot growth. The way gibberellins are made in corn will be studied using combination of chemical and genetic experimental approaches. The work is important because it will lead to a greater understanding of how plant growth is controlled. This work has both basic and practical importance and may eventually lead to increased crop productivity and an increase in the world's food supplies.