Gibberellin Biosynthesis in Maize-Chemistry, Biochemistry and Genetics

本实验室的总体研究目标是了解赤霉素（gibberellin， GA）依赖性玉米茎部生长的生化、遗传和分子基础。该项目对科学进步非常重要，因为研究结果确定了玉米生长的控制因素，玉米是一种重要的社会福利经济植物。赤霉素是一种植物激素，控制着茎的生长。它们存在于整个植物界。首席研究员（PI）率先使用单基因突变来揭示ga生物合成途径的细节；这些突变体也被用来评价特定赤霉素在控制生长方面的生物学意义。该计划将继续对玉米赤霉素的研究，这将(i)定义特定玉米赤霉素的生物合成起源和代谢，（ii）定位被矮突变体（ga -突变体）阻断的途径中的特定步骤，（iii）筛选转座子标记的ga -矮突变体，目的是克隆和表达控制玉米生物合成途径步骤的基因。本项目研究了赤霉素合成的生物学途径，赤霉素是调节芽部生长的植物激素。将采用化学和基因实验相结合的方法研究玉米中赤霉素的合成方法。这项工作很重要，因为它将使人们更好地了解植物生长是如何被控制的。这项工作具有基础和实际的重要性，并可能最终导致提高作物生产力和增加世界粮食供应。

9604460 Phinney The overall research goal of this laboratory is to understand the biochemical, genetic and molecular basis for gibberellin (GA)-dependent shoot growth in maize. The program is important to the advancement of science in that the results define control elements for the growth of maize, an important economic plant for the welfare of society. Gibberellins are plant hormones that are controllers of shoot growth. They are present throughout the plant kingdom. The Principal Investigator (PI) has pioneered the use of single gene mutants to unravel the details of the GA-biosynthetic pathway; these mutants have also been used to evaluate the biological significance of specific gibberellins in their control of growth. The projected program will continue studies on the gibberellins of maize which will (i) define the biosynthetic origin and metabolism of specific maize gibberellins, (ii) locate specific steps in the pathway that are blocked by dwarf mutants (GA-mutants) and (iii) screen for transposon tagged GA-dwarf mutants with a goal of cloning and expressing the genes that control steps in the maize biosynthetic pathway. This project addresses the biological routes for synthesis of gibberellins, plant hormones that regulate shoot growth. The way gibberellins are made in corn will be studied using combination of chemical and genetic experimental approaches. The work is important because it will lead to a greater understanding of how plant growth is controlled. This work has both basic and practical importance and may eventually lead to increased crop productivity and an increase in the world's food supplies.