Community Dynamics Localization and Fixation Potentials of Marine N2-Fixing Microbial Assemblages

海洋固氮微生物群落的群落动态定位和固定潜力

• 批准号: 8820304
• 负责人: Randall Alberte
• 金额: $ 12.89万
• 依托单位: University of Chicago
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-15 至 1991-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8820304&HistoricalAwards=false
• 关键词: Community; Dynamics; Localization; Fixation; Potentials

Chronic combined nitrogen deficiencies characterize vast segments of the world's oceans. Microbial nitrogen fixation helps to alleviate these deficiencies. The role of eubacteria as nitrogen fixers has received less attention than cyanobacteria. This project focuses on the role of eubacteria in N-depleted North Carolina coastal waters using recently developed immunological and molecular techniques to provide a cell specific set of fluorescence and isotopic assays of bacterial population and community dynamics in benthic and planktonic habitats. Such techniques and assays allow for identification of cells competent and induced to fix N, and provide a means to detect the presence of nitrogenase genes in total population DNA. These techniques will allow (1) direct, quantitative information on microbial N fixation potentials and sites thereof, (2) assessment of environmental conditions that regulate nitrogenase gene expression, and (3) evaluation of recently described, numerically and trophically important picoplanktonic cyanobacteria for their N fixing capabilities.

慢性复合氮缺乏症的特点是 世界海洋的一部分。微生物固氮 有助于弥补这些不足。 真细菌的作用 因为固氮剂受到的关注比 蓝细菌。 这个项目的重点是真细菌的作用 在氮耗尽的北卡罗来纳州沿海沃茨，最近使用 发展了免疫学和分子技术， 细胞特异性荧光和同位素测定 细菌种群和群落动态 南极栖息地。 这样的技术和测定允许 鉴定感受态细胞和诱导固定N的细胞，和 提供了一种检测固氮酶基因存在的方法， 总人口DNA。 这些技术将允许（1）直接， 微生物固氮潜力的定量信息， （2）环境条件评价， 调节固氮酶基因表达，和（3）评价 最近描述的，数量和营养重要 picoacetonic蓝藻的固氮能力。