Acquisition of a Phase-Lifetime Spectrofluorometer

获得相位寿命分光荧光计

• 批准号: 8820594
• 负责人: Lawrence C. Kuo
• 金额: --
• 依托单位: Trustees of Boston University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-06-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8820594&HistoricalAwards=false
• 关键词: Acquisition; Phase; Lifetime; Spectrofluorometer

Trytophan and tyrosine are natural fluorophores and components of proteins. These biological chromophores are fluorescent, i.e., when photo excited they emit light which is characteristic of their chemical and physical environment. Fluorescence is an important analytical technique because of its extreme sensitivity and specificity. The time scale of the fluorescence phenomenon can make the technique a very effective probe of the surroundings of the fluorophore. The requested spectrofluorometer is a state-of-the-art instrument which provides spectral differentiation of fluorophores in a mixture on the basis of difference in fluorescence lifetimes. In the case of a protein molecule, the true emission profile of the tyrosine and tryptophan residues which contribute to the total fluorescence of the protein can be identified and monitored. The spectrofluorometer can also offer information on the molecular size, conformation, and interactions of fluorophores in a protein molecule. With these measurements, the dynamic chemical and physical nature of the fluorophores can be deduced. The biological research projects which require the use of the spectrofluorometer include: 1. The conformation of a metabolic enzyme, ornithine transcarbamoylase, under the influence of a variety of ligands will be analyzed. The goal is to probe the possible metabolic regulation of this enzyme. One of the most challenging problems in biochemistry today is the study of metabolic regulation. 2.The characterization of chromophore-based biopolymers using fluorescence techniques will be conducted to provide insight into how groups are assembled and communicate electronically along a polymer backbone. This study will aid the elucidation of the structure of biological polymer composite and the design of new materials for optical and electronic processing. 4. The molecular mechanism of the retinal based proteins, rhododpsin and bacteriorhodopsin, will be investigated for the understanding of phototransduction in membranes. 5. The development of skeletal muscle tissue in the vertebrate embryo is a complex multistep process which is not yet well understood. The sensitivity of the spectrofluorometer will be exploited to dissect out the signal transduction cascade within the myoblast membrane for the understanding of cell differentiation of muscle.

色氨酸和酪氨酸是天然的荧光团和组分 蛋白质。 这些生物发色团是荧光的， 也就是说，当光激发时，它们发出光， 其化学和物理环境的特点。 荧光是一种重要的分析技术， 它的极端敏感性和特异性。 的时间尺度 荧光现象可以使该技术非常有效 荧光团周围的探针。 请求的 荧光分光光度计是一种最先进仪器， 提供混合物中荧光团的光谱差异 基于荧光寿命的差异。 在 在蛋白质分子的情况下， 酪氨酸和色氨酸残基，它们对总的 可以识别和监测蛋白质的荧光。 荧光分光光度计还可以提供有关 荧光团的分子大小、构象和相互作用 在蛋白质分子中。 通过这些测量， 荧光团的化学和物理性质可以 推导 生物研究项目需要使用 荧光分光光度计包括： 1.一种代谢酶--鸟氨酸的构象 在多种配体的影响下， 将被分析。 我们的目标是探索可能的代谢 这种酶的调节。 最具挑战性的一 当今生物化学中的一个问题是研究代谢 调控 2.基于生色团的生物聚合物的表征 将进行荧光技术， 群体是如何通过电子方式聚集和交流的 沿着聚合物主链。 这项研究将有助于阐明 生物高分子复合材料的结构和设计 用于光学和电子加工的新材料。 4.视网膜基蛋白的分子机制， 视紫红质和细菌视紫红质，将被调查的 了解膜中的光传导。 5.脊椎动物骨骼肌组织的发育 胚胎发育是一个复杂的多步骤过程， 明白荧光分光光度计的灵敏度为 利用它来剖析细胞内的信号转导级联反应， 成肌细胞膜对于理解细胞 肌肉的分化。