Development of measurement of intracellular ion concentration using a phase-resolved fluorescence lifetime method for multicomponent

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Calcium ion concentration was measured with phase-resolved fluorescence lifetime method. Fluorescence dye Calcium Orange was used. The fluorescence lifetime was changed when the concentration of calcium ion was varied from10 to 2000nM. In order to improve the quantitativity of the calcium ion measurement, we investigated a fluorescence lifetime of calcium orange in calcium ion solution including protein. As a result, the fluorescence lifetime does not depend on the protein concentration range from 40 to 80 mg/ml, which is same as that in a cell.