Acquisition of Shared Spectropolarimeter for CD Studies

这项建议要求得到支持，以获得强大和高度 灵敏的CD光谱设备。 的所有项目 利用重组DNA技术， 重组蛋白质类（神经生长因子），转羧酶 亚基、葡萄糖激酶和逆转录病毒核衣壳蛋白。 我们建议确定这些的结构和功能 重组蛋白，并将它们与相应的 天然蛋白质的性质;很明显， 必须先评估属性，然后才能 翻译。 这些研究需要CD谱的比较 从天然和重组蛋白质，以及功能 问题研究 因此我们需要一台能够 高灵敏度、低波长的定量评价 少量样品（通常是天然蛋白质）。 灵敏度对于核衣壳的监测也很重要 蛋白质与核酸的结合， 核酸的CD带。 当前访问请求的 校园里没有CD仪器，因为居民 分光偏振计已经过时，不适用。 请求的 仪器将可供任何其他合格用户使用， 校园，时间允许。

This proposal requests support to obtain powerful and highly sensitive CD spectroscopic equipment. All of the projects involve the use of recombinant DNA technology to generate recombinant proteins (nerve growth factor), transcarboxylase subunits, glucokinase, and the retroviral nucleocapsid protein. We propose to determine the structure and function of these recombinant proteins and to compare them with the corresponding properties of the native protein; it is clear that the structural properties must be evaluated before any functional changes can be interpreted. Such studies require the comparison of CD spectra from native and recombinant proteins, as well as functional studies. Thus we require access to a spectropolarimeter capable of high sensivity and low wavelength for quantitative evaluation of small amounts of sample (frequently of the native protein). Sensitivity is also important for the monitoring of nucleocapsid protein binding to nucleic acid by following the amplitude of the CD band from the nucleic acid. Currently access to the requested CD instrumentation is unavailable on campus, because the resident spectropolarimeter is out of date and inadequate. The requested instrument will be available to serve any other qualified user on campus, time permitting.