Acquisition of a Transmission Electron Microscope

加州州立大学长滩分校已经申请了相应的资金，用于建造一台透射电子显微镜(TEM)。该仪器将由自然科学学院内的一些研究人员用于各种需要高分辨率的明暗场成像和选定区域衍射的项目。这些项目包括研究在载体珠上培养的原代无脊椎动物细胞的细胞学去分化，研究培养的软体动物细胞中血蓝蛋白和无机聚磷酸盐包裹体的形成和分泌，检测各种纯化的中间丝蛋白在体内重组为丝的能力，以及检测纯化的、可溶的神经蛋白寡聚体的大小和几何形状。其他需要常规透射电子显微镜的项目包括一项定量确定胞间连丝和连丝小管通讯的研究，以估计光合作用产物共形体和质外体运输到筛管的相对程度。该仪器还将用于研究卡氏肺孢子虫包囊和滋养体形态的发育变化，并建立该生物与宿主肺上皮细胞的附着模式。许多研究人员需要该仪器进行免疫细胞学研究。这些研究旨在确定海洋无脊椎动物在暴露于环境应激时金属硫蛋白和热休克70蛋白的诱导和细胞学分布。其他研究包括磷酸蛋白磷酸酶-1激活激酶的免疫定位，以及参与糖原代谢的酶系中的各种磷酸酶及其调节亚单位。电子显微镜结合氧气等离子体微焚烧和能量色散X射线微区分析也将被用于确定在商业和生态上重要的海洋无脊椎动物中参与金属积累的超微结构特征和细胞配体。

Matching funds have been requested by California State University Long Beach for a transmission electron microscope (TEM). The instrument will be used by a number of investigators within the School of Natural Sciences for a diverse range of projects requiring high resolution bright and dark field imaging and selected area diffraction. These projects include studying the cytological dedifferentiation of primary invertebrate cells cultured on support beads, studying the formation and secretion of haemocyanin and inorganic polyphosphate inclusions in cultured molluscan cells, examining the ability of various purified intermediate filament proteins to be reconstituted into filaments in vivo and examining the size and geometry of purified, soluble neuroprotein oligomers. Other projects requiring conventional TEM include a study to quantitatively determine plasmodesmatal and desmotubule communication to estimate the relative degree of involvement of symplastic and apoplastic transport of photosynthates to sieve tubes. The instrument will also be used to study the developmental changes in the cysts and trophozoite forms of Pneumocystis carinii and establish the mode of attachment of the organism to host lung epithelial cells. A number of investigators require the instrument for immunocytological studies. These studies aim to determine the induction and cytological distribution of metallothionein and heat shock 70 proteins in marine invertebrates upon exposure to environmental stress. Other studies include the immunolocalization of phosphoprotein phosphatase-1 activating kinase and the various phosphatases and their regulatory subunits in the enzyme cascade involved in glycogen metabolism. TEM in combination with O2 plasma microincineration and energy dispersive X-ray microanalysis will also be used to determine the ultrastructural features and cellular ligands involved in metal accumulation in commercially and ecologically important marine invertebrates.