Lab-on-CMOS Electrochemical Microsystem for High Throughput Characterization of Membrane Proteins

用于膜蛋白高通量表征的 CMOS 实验室电化学微系统

• 批准号: 1307939
• 负责人: Andrew Mason
• 金额: $ 36万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1307939&HistoricalAwards=false
• 关键词: Lab; CMOS; Electrochemical; Microsystem; Throughput

Membrane proteins are the essential components of the cell that connect all living organisms to the outside world. The mechanisms by which various membrane proteins impart vital functions to natural bilayer lipid membranes (BLMs) are poorly understood, particularly at the molecular and atomic scales. Although there is a critical need to understand the structure and function of novel membrane proteins, discoveries are limited by the substantial bottleneck of preparing and executing detailed experimental studies. Objective: The goal of this interdisciplinary project is to establish new technologies and methodologies for coupling microelectronics with biological interfaces that will enable high throughput characterization of membrane proteins. To achieve this goal, we propose to design a lab-on-CMOS platform that integrates a 1000-element array of microfluidic planar-BLM chambers with embedded CMOS electrochemical instrumentation circuits. The proposed approaches will overcome significant technical barriers and contribute new mechanisms for silicon-to-protein communication pathways, enabling new directions for future research activities and commercial products. Intellectual Merit: The proposed approaches for coupling microelectronics with biological interfaces would overcome significant technical barriers and contribute new mechanisms for silicon-to-protein communication pathways, enabling a new direction for future research activities and commercial products. These approaches will be incorporated into the proposed model instrument which will itself provide ground-breaking new capabilities for high throughput biomembrane characterization enabling significant subsequent advances in many fields of biology, nanomaterials, and medicine. The research efforts would also advance scientific and engineering knowledge in two primary areas.Broader Impact: A model instrument will be developed with new capabilities for high throughput biomembrane characterization, enabling significant subsequent advances in many fields that could transform the frontiers of knowledge, advance our fundamental understanding of basic biological processes, and aid in the generation of new, engineered membrane proteins for sensor platforms, high throughput drug screening, and development of new medical diagnostics and disease treatments, all with wide-reaching benefits to society. Results of the multidisciplinary research will be integrated into education by direct involvement of graduate and undergraduate student researchers, development and delivery of a precollege STEM presentation, and participation in a novel project-based MultidisciplinaryBioprocessing Laboratory course. In addition, research experience will be provided for a secondary education science teacher who will integrate the experience into a teaching module for K-12 programs, including opportunities that target students from underrepresented groups. The results of this project will be disseminated through journal publications, conference presentations, and websites of the investigators and their associated research centers.

膜蛋白是连接所有生物与外部世界的细胞的重要组成部分。各种膜蛋白赋予天然双层类脂膜(BLM)重要功能的机制尚不清楚，特别是在分子和原子水平上。尽管迫切需要了解新型膜蛋白的结构和功能，但由于准备和执行详细的实验研究的实质性瓶颈，这些发现受到了限制。目的：这一跨学科项目的目标是建立微电子学与生物界面相结合的新技术和方法，使膜蛋白的高通量表征成为可能。为了实现这一目标，我们建议设计一个集成了1000个微流控平面BLM室阵列和嵌入式CMOS电化学仪器电路的Lab-on-Cmos平台。建议的方法将克服重大的技术障碍，并为硅到蛋白质的通信途径提供新的机制，为未来的研究活动和商业产品提供新的方向。智力优势：拟议的微电子与生物接口耦合的方法将克服重大技术障碍，并为硅到蛋白质的通信途径提供新的机制，为未来的研究活动和商业产品提供新的方向。这些方法将被整合到拟议的模型仪器中，该模型仪器本身将为高通量生物膜表征提供突破性的新能力，使其能够在生物学、纳米材料和医学的许多领域取得重大后续进展。这些研究工作还将促进两个主要领域的科学和工程知识。更广泛的影响：将开发具有高通量生物膜表征新能力的模型仪器，使随后许多领域的重大进展能够改变知识的前沿，增进我们对基本生物过程的基本理解，并有助于为传感器平台生成新的工程膜蛋白，高通量药物筛选，以及开发新的医疗诊断和疾病治疗，所有这些都将给社会带来广泛的好处。多学科研究的结果将通过研究生和本科生研究人员的直接参与、开发和提供大学前STEM演示文稿以及参与基于项目的新的多学科生物处理实验室课程来整合到教育中。此外，将为一名中学教育科学教师提供研究经验，他将把这些经验纳入K-12方案的教学模块，包括针对代表性不足群体的学生的机会。该项目的成果将通过期刊出版物、会议报告和调查人员及其相关研究中心的网站进行传播。