Equipment Upgrading of Microcomputer Workstation

我们建议对现有的微型计算机技术进行升级。DNA-蛋白相互作用是我们理解DNA复制、神经元-胶质细胞分化和乳腺上皮细胞分化调控机制的关键。我们需要一个功能强大的计算机系统来分析数据并以图形方式呈现结果。计算机将用于以下项目：a)枯草芽孢杆菌DNA复制的分子生物学，研究染色体和质粒复制的膜结合作用。b)利用大鼠神经元-胶质干细胞衍生细胞类型进行神经元-胶质分化的分子生物学研究，频率约为10-5。其中两种是神经元样细胞类型，第三种是胶质样细胞类型。c)分析生长因子对乳腺癌细胞系生长分化的影响。

We propose to upgrade our current technical microcomputer. DNA- protein interactions are the key for our understanding of the mechanism of regulations of DNA replication, neuronal-glial differentiation and mammary epithelial differentiation. We need a powerful computer system to analyze data and present the results graphically. The computer will be used in the following projects: a) Molecular biology of DNA replication in Bacillus subtilis which studies the role of membrane association of chromosomal and plasmid replication. b) Molecular biology of neuronal-glial differentiation using a rat neuronal-glial stem derivative cell types with an approximate frequency of 10-5. Two of them are neuronal-like cell types and the third is a glial- like cell type. c) Analysis of the effect of growth factors on the growth and differentiation of mammary carcinoma cell lines.