Neuron-glia Differentiation of a Stem Cell Line; RT4

干细胞系的神经元-胶质细胞分化；

• 批准号: 9630813
• 负责人: Noboru Sueoka
• 金额: $ 4万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9630813&HistoricalAwards=false
• 关键词: Neuron; glia; Differentiation; Stem; Cell

IBN: 9630813 PI: Sueoka The nervous system of higher organisms consists of neurons and glial cells; the function of glial cells is to help neurons. These two cell types are known to come from common, embryonic precursor cells (neural stem cells). The differentiation of neural stem cells into neurons and glial cells, therefore, is the critical process for the development of the nervous system. In spite of the fundamental importance of stem cell differentiation, the mechanisms underlying this differentiation (identification of the genes involved and the functions of these genes) are not well understood. We have isolated and characterized in detail a rat stem cell line that differentiates into neurons and glial cells in culture. This system is ideal for molecular studies on the neuronal-glial differentiation of neural stem cells. Using this system, we have isolated several candidate gene fragments that may be critically important for neural stem cell differentiation. Using these fragments, we plan to isolate and characterize the genes and determine their functions in the development of the nervous system. Among several gene fragments that are potentially important, there is a particularly promising gene fragment which we are going to study first. The gene corresponding to this fragment is expressed in the neuronal cell line but not in the stem cell line. This fragment has a region with almost identical DNA sequence to a control region of a Drosophila gene that is essential for the development of the nervous system.

IBN：9630813 PI：Sueoka 高等生物的神经系统由神经元和神经胶质细胞组成；神经胶质细胞的功能是帮助神经元。已知这两种细胞类型来自常见的胚胎前体细胞（神经干细胞）。因此，神经干细胞分化为神经元和神经胶质细胞是神经系统发育的关键过程。尽管干细胞分化具有根本重要性，但这种分化的机制（所涉及基因的鉴定以及这些基因的功能）尚不清楚。我们分离并详细描述了一种大鼠干细胞系，该干细胞系在培养物中分化为神经元和神经胶质细胞。该系统非常适合神经干细胞神经元-胶质细胞分化的分子研究。使用该系统，我们分离出了几个对于神经干细胞分化可能至关重要的候选基因片段。利用这些片段，我们计划分离和表征这些基因，并确定它们在神经系统发育中的功能。在几个可能重要的基因片段中，有一个特别有前途的基因片段是我们首先要研究的。与该片段相对应的基因在神经元细胞系中表达，但在干细胞系中不表达。该片段的一个区域的 DNA 序列与果蝇基因的控制区域几乎相同，该区域对于神经系统的发育至关重要。