Methodology for Studies of the Regulation of Adrenal Steroidogenesis

研究，包括研究野鸭胚胎中肾上腺类固醇生成的调节方法的发展，将得到支持。这些研究将最终有助于我们了解与肾上腺类固醇生成功能的整体控制有关的调节机制。实验系统为野鸭胚胎肾上腺发育过程。组织化学和免疫细胞化学分析，加上超微结构分析，是必要的，以确定产品和确定神经组织的分布。组织灌流技术和放射免疫分析是确定发育组织的功能特性所必需的。该方法将允许研究者确定何时发育中的原基对促肾上腺皮质激素和血管紧张素II变得敏感。

Studies, involving the development of methods for studying the regulation of adrenal steroidogenesis in the mallard duck embryo, will be supported. The studies will ultimately contribute to our understanding of the regulatory mechanisms concerned with the overall control of the adrenal steroidogenic function. The experimental system will be the developing adrenal gland in the mallard duck embryo. Histochemical and immunocytochemical analysis, coupled with ultrastructure analysis, are necessary to identify the products and identify the distribution of nerve tissue. Tissue-superfusion technique and radioimmunoassay are needed for defining the functional characteristics of the developing tissue. The approach will allow the investigators to establish when the developing anlage becomes sensitive to corticotropin and angiotensin II.