Methodology for Studies of the Regulation of Adrenal Steroidogenesis

肾上腺类固醇生成调节的研究方法

• 批准号: 8820923
• 负责人: James Cronshaw
• 金额: --
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-01-01 至 1991-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8820923&HistoricalAwards=false
• 关键词: Methodology; Studies; Regulation; Adrenal; Steroidogenesis

Studies, involving the development of methods for studying the regulation of adrenal steroidogenesis in the mallard duck embryo, will be supported. The studies will ultimately contribute to our understanding of the regulatory mechanisms concerned with the overall control of the adrenal steroidogenic function. The experimental system will be the developing adrenal gland in the mallard duck embryo. Histochemical and immunocytochemical analysis, coupled with ultrastructure analysis, are necessary to identify the products and identify the distribution of nerve tissue. Tissue-superfusion technique and radioimmunoassay are needed for defining the functional characteristics of the developing tissue. The approach will allow the investigators to establish when the developing anlage becomes sensitive to corticotropin and angiotensin II.

研究将支持研究，涉及研究用于研究绿鸭胚胎中肾上腺类固醇生成的方法的研究。这些研究最终将有助于我们理解与肾上腺类固醇功能总体控制有关的调节机制。实验系统将是野鸭胚胎中发育的肾上腺。组织化学和免疫细胞化学分析，再加上超微结构分析，对于识别产物并确定神经组织的分布是必要的。需要组织灌注技术和放射免疫分子来定义发育组织的功能特征。该方法将允许研究人员确定何时发育范围对皮质激素和血管紧张素II敏感。