Equipment for Undergraduate Plant Physiology and Molecular Biology Laboratory Course

授予密歇根州立大学植物学和植物病理学系的这一奖项将提供资金，为现代植物生理学和分子生物学的新的高级本科实验室课程购买设备。密歇根州立大学目前没有开设这样的课程，尽管有许多学生学习不同类型的植物科学，他们可以从这样的课程中受益。新课程将取代目前属于两门高级植物生理学课程的两个实验室；这些实验室将继续作为讲座课程。该合同提供资金用于购买8种设备：1台高速离心机和转子、6台凝胶电泳仪、2台旋转蒸发器、4台pH计、1台扫描分光光度计、2台摇水浴、2台蛋白质和DNA印迹设备以及1台闪烁计数器。这些课程将被用来进行实验室会议，专门讨论农杆菌介导的基因转移；细菌转化；植物DNA、RNA和蛋白质的纯化、电泳和转移印迹，以及通过与DNA探针和抗体杂交进行检测；植物激素诱导基因表达；酶分析；以及植物激素的酶免疫分析。目前植物学和植物病理学系现有的教学设备对拟议的课程来说是非常不足的。接受资助的机构正在将这一NSF奖项与非联邦来源的资金相匹配。

This award to the Department of Botany and Plant Pathology at Michigan State University will provide funds to buy equipment for a new advanced undergraduate laboratory course in modern plant physiology and molecular biology. Such a course is not currently offered at MSU, even though there are many students studying diverse types of plant science who could benefit from such a course. The new course will replace two laboratories that are currently part of two advanced plant physiology courses; these will continue as just lecture courses. The award provides funds to buy eight types of equipment: one high-speed centrifuge and rotors, six gel electrophoresis units, two rotary evaporators, four pH meters, one scanning spectrophotometer, two shaking water baths, two protein and DNA blotting apparatuses, and one scintillation counter. These will be used to conduct laboratory sessions devoted to Agrobacterium-mediated gene transfer; bacterial transformation; purification, electrophoresis, and transfer blotting of plant DNA, RNA, and proteins, and detection by hybridization with DNA probes and antibodies; induction of gene expression by plant hormones; enzyme assays; and enzyme- linked immunoassay of plant hormones. The current available teaching equipment in the Department of Botany and Plant Pathology is very inadequate for the proposed course. The grantee institution is matching this NSF award with funds from non-Federal sources.