Equipment for Undergraduate Plant Physiology and Molecular Biology Laboratory Course

本科生植物生理学与分子生物学实验课程设备

• 批准号: 8851155
• 负责人: Jonathan Walton
• 金额: $ 2.48万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-15 至 1991-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8851155&HistoricalAwards=false
• 关键词: Equipment; Undergraduate; Plant; Physiology; Molecular

This award to the Department of Botany and Plant Pathology at Michigan State University will provide funds to buy equipment for a new advanced undergraduate laboratory course in modern plant physiology and molecular biology. Such a course is not currently offered at MSU, even though there are many students studying diverse types of plant science who could benefit from such a course. The new course will replace two laboratories that are currently part of two advanced plant physiology courses; these will continue as just lecture courses. The award provides funds to buy eight types of equipment: one high-speed centrifuge and rotors, six gel electrophoresis units, two rotary evaporators, four pH meters, one scanning spectrophotometer, two shaking water baths, two protein and DNA blotting apparatuses, and one scintillation counter. These will be used to conduct laboratory sessions devoted to Agrobacterium-mediated gene transfer; bacterial transformation; purification, electrophoresis, and transfer blotting of plant DNA, RNA, and proteins, and detection by hybridization with DNA probes and antibodies; induction of gene expression by plant hormones; enzyme assays; and enzyme- linked immunoassay of plant hormones. The current available teaching equipment in the Department of Botany and Plant Pathology is very inadequate for the proposed course. The grantee institution is matching this NSF award with funds from non-Federal sources.

该奖项授予植物学和植物病理学系， 密歇根州立大学将提供资金购买设备， 现代工厂高级本科实验新课程 生理学和分子生物学。 这样的课程目前还没有 在MSU提供，即使有很多学生学习 不同类型的植物科学谁可以受益于这样一个 当然了 新课程将取代两个实验室， 目前两个先进的植物生理学课程的一部分;这些 将继续作为讲座课程。 该奖项提供资金 购买八种设备：一台高速离心机， 转子，六个凝胶电泳单元，两个旋转蒸发器， 四个pH计，一个扫描分光光度计，两个振荡水 浴，两个蛋白质和DNA印迹装置，和一个 闪烁计数器 这些将用于进行实验室 专门讨论农杆菌介导的基因转移的会议; 细菌转化;纯化，电泳，和 植物DNA、RNA和蛋白质的转移印迹和检测 通过与DNA探针和抗体杂交;诱导 植物激素的基因表达;酶分析;和酶- 植物激素的联用免疫测定。 当前可用 植物学系教学设备 病理学对这门课程来说是非常不够的。 的 受资助机构正在将这个NSF奖项与来自 非联邦来源