Equipment for a Modern Undergraduate Laboratory in Plant Biology

该项目提供现代化的实验室设备， 建立现代植物细胞与分子科学教学 程序. 两个高级实验室课程受到影响： 生理学和植物生长发育。 植物生理学 目前正在进行全面翻新的手中， 首席研究员（一年前被聘为 在现代植物实验室科学部门的空白）。 植物生长 发展是一门新课程，1987年首次开设， 1988. 该设备是在一个完整的两个实验室课程， 让学生观察植物的基本原理的方式 生物学和获得经验的方法，目前采用的 植物科学实验室研究 作为这项赠款的直接结果， 学生有机会利用以下技术 在他们对植物过程的研究：荧光和气体交换， 全叶/全株水平，细胞器分离和 表征，亚细胞器分级，极谱气体分析 在溶液中，蛋白质的电泳分离和定量 通过扫描密度测定法、免疫化学测定法，包括蛋白质印迹法， 和酶联免疫吸附测定以及体外翻译。 植物分子和细胞生物学目前是研究的热点领域。 研究工作正在产生很大的兴奋。 一个 这个项目的目标是将一些刺激传递给 地方一级 一些设备也被用于其他 生物学专业的高级实验室课程，其中大多数人 继续他们的正规教育，无论是研究生或专业 学校 受赠机构正在将NSF奖项与同等奖项相匹配 从非联邦来源获得的金额。

This project is providing modern laboratory equipment to establish a contemporary cell and molecular plant science teaching program. Two upper-level laboratory courses are affected: Plant Physiology, and Plant Growth and Development. Plant Physiology currently is undergoing complete renovation at the hands of the principal investigator (who was hired a year ago to fill the department's void in modern plant laboratory sciences). Plant Growth and Development is a new course, offered for the first time in 1987- 1988. The equipment is fitting out two entire laboratory courses in a manner that allows students to observe fundamental principles in plant biology and to gain experience in methodologies currently employed in plant science laboratory research. As direct result of this grant, students are having an opportunity to utilize the following techniques in their study of plant processes: fluorescence and gas exchange at the whole leaf/whole plant level, organella isolation and characterization, suborganella fractionation, polarographic gas assay in solution, electrophoretic separation of proteins and quantification by scanning densitometry, immunochemical assays including Western blots and enzyme-linked immunosorbant assays, and in vitro translations. Plant molecular and cell biology currently are areas of intense research efforts which are generating a great deal of excitement. An objective of this project is to transmit some of that stimulation to the local level. Some of the equipment also is being used in other upper-level laboratory courses by biology majors, a majority of whom continue their formal education in either graduate or professional schools. The grantee institution is matching the NSF award with an equal sum obtained from non-Federal sources.