Introduction of Modern MS into the Upper Division Undergraduate Chemistry Curriculum

化学（12）智力价值：该项目的主要目标是：1)通过为学生提供更直接的领域的实验，改善本科分析化学和物理化学的教学和实验室经验；2)激发他们对现代化学的兴趣。质谱（MS）已成为后基因组时代的主要分析技术之一。蛋白质和小分子的质谱分析有望使分子医学、分子毒理学、保护环境和促进对生物恐怖主义的反应发生革命性的进展。在改革我们的高年级本科生实验课程时，我们因此向学士和理学士学生引入了MS。正在进行的实验改编自教育和研究文献。分析化学实验室（chem304）正在增加使用液相色谱串联质谱（LC-MS/MS）来鉴定未知蛋白质。物理化学实验室（CHEM 305/322）正在增加使用质谱/质谱分析碰撞诱导解离。该仪器也可用于其他实验课程和本科生研究项目。新的实验将在三年的时间内实施，在第一年我们的理学士专业和化学以外的教师顾问团队的帮助和反馈下。更广泛的影响：在本科阶段提供MS是一种创新的方式，让学生为跨学科的科学未来做好准备。将质谱实验纳入实验课程对学生学习的影响通过直接问卷调查和关于质谱实验本身的退出访谈来评估，并且通常通过比较第一年（在质谱实验对他们实施之前）的控制ba专业组的测试表现和学生兴趣与随后两年的这些数据来评估。我们相信学生们会因为他们的经历而对化学和科学的职业生涯更加兴奋，我们希望将这一努力的预期成功传达给其他本科项目。

Chemistry (12)Intellectual merit: The major objectives of this project are 1) to improve the teaching and laboratory experience in undergraduate analytical and physical chemistry by providing experiments in areas of greater immediacy to students and 2) to stimulate their interests in modern chemistry. Mass spectrometry (MS) has become one of the major analytical techniques in the post-genomic era. Mass spectrometric analysis of both proteins and small molecules promises molecular advances that will revolutionize molecular medicine, molecular toxicology, and efforts to safeguard the environment and facilitate responses to bioterrorism. In revamping our upper division undergraduate laboratory curriculum, we therefore are introducing MS to both B.A. and B.S. students. The experiments being developed are adapted from the educational and research literature. The analytical chemistry laboratory (CHEM 304) is adding of the use of liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify an unknown protein. The physical chemistry laboratory (CHEM 305/322) is adding the use of MS/MS to analyze a collision induced dissociation. The instrument is also available for other laboratory courses and for undergraduate research projects. The new experiments are being implemented over a three-year period, with the assistance of and feedback from our B.S. majors in the first year and a team of faculty consultants outside chemistry.Broader impacts: Making MS accessible at the undergraduate level is an innovative way to prepare students for the interdisciplinary future of science. The impact on student learning of incorporating the MS experiments in the laboratory courses is being evaluated by directed questionnaires and exit interviews regarding the MS experiments themselves, and in general by comparing test performances and student interest among the control B.A. major group in the first year (before the MS experiments have been implemented for them) to these data in the subsequent two years. We believe that students will become more excited about careers in chemistry and science in general as a consequence of their experience, and we expect to convey the projected success of this endeavor to other undergraduate programs.