Introduction of Modern MS into the Upper Division Undergraduate Chemistry Curriculum

化学（12）智力价值：本项目的主要目标是1）通过为学生提供更直接的实验，改善本科分析和物理化学的教学和实验室经验，2）激发他们对现代化学的兴趣。质谱（MS）已成为后基因组时代的主要分析技术之一。蛋白质和小分子的质谱分析预示着分子的进步，这将彻底改变分子医学，分子毒理学，以及保护环境和促进应对生物恐怖主义的努力。在改革我们的高年级本科实验室课程，因此，我们正在引入MS的学士学位。和B. S。学生正在开发的实验改编自教育和研究文献。 分析化学实验室（CHEM 304）正在增加液相色谱串联质谱法（LC-MS/MS）的使用，以鉴定未知蛋白质。物理化学实验室（CHEM 305/322）正在增加使用MS/MS来分析碰撞诱导解离。该仪器也可用于其他实验室课程和本科研究项目。在我们的B.S.的协助和反馈下，新的实验将在三年内实施。更广泛的影响：使MS在本科阶段可获得是一种创新的方式，为学生准备科学的跨学科未来。将MS实验纳入实验室课程对学生学习的影响正在通过针对MS实验本身的定向问卷调查和退出访谈进行评估，并通过比较控制BA之间的测试表现和学生兴趣。主要群体在第一年（在MS实验已经为他们实施之前），这些数据在随后的两年。我们相信，学生会变得更加兴奋的职业生涯在化学和科学一般作为他们的经验的结果，我们希望传达这一奋进的预期成功，以其他本科课程。

Chemistry (12)Intellectual merit: The major objectives of this project are 1) to improve the teaching and laboratory experience in undergraduate analytical and physical chemistry by providing experiments in areas of greater immediacy to students and 2) to stimulate their interests in modern chemistry. Mass spectrometry (MS) has become one of the major analytical techniques in the post-genomic era. Mass spectrometric analysis of both proteins and small molecules promises molecular advances that will revolutionize molecular medicine, molecular toxicology, and efforts to safeguard the environment and facilitate responses to bioterrorism. In revamping our upper division undergraduate laboratory curriculum, we therefore are introducing MS to both B.A. and B.S. students. The experiments being developed are adapted from the educational and research literature. The analytical chemistry laboratory (CHEM 304) is adding of the use of liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify an unknown protein. The physical chemistry laboratory (CHEM 305/322) is adding the use of MS/MS to analyze a collision induced dissociation. The instrument is also available for other laboratory courses and for undergraduate research projects. The new experiments are being implemented over a three-year period, with the assistance of and feedback from our B.S. majors in the first year and a team of faculty consultants outside chemistry.Broader impacts: Making MS accessible at the undergraduate level is an innovative way to prepare students for the interdisciplinary future of science. The impact on student learning of incorporating the MS experiments in the laboratory courses is being evaluated by directed questionnaires and exit interviews regarding the MS experiments themselves, and in general by comparing test performances and student interest among the control B.A. major group in the first year (before the MS experiments have been implemented for them) to these data in the subsequent two years. We believe that students will become more excited about careers in chemistry and science in general as a consequence of their experience, and we expect to convey the projected success of this endeavor to other undergraduate programs.