Introduction of Molecular Genetics Techniques into the Undergraduate Curriculum

本项目的目标是将当前的技术引入 分子遗传学纳入本科生物学课程。 的 它提供的设备使它有可能提供一个新的实验室， 分子生物学课程，以及改善目前的细胞 生物学、微生物学和普通生物学课程实验室。 每年约有170名学生，在所有本科层次，将 受到这些变化的影响。 新设备包括细菌培养箱和水 培养细菌以分离质粒的水浴振荡器; CO2 培养箱和层流洁净工作台，供学生使用纸巾工作 用于DNA/RNA纯化和浓缩的微量离心机; 水平琼脂糖凝胶单元和DNA检测照相机系统， 分离和分析限制性内切酶的产物; 聚丙烯酰胺平板凝胶装置，用于分析体外产物 转录反应;检测细胞的荧光照明器- 转移基因产物的表面表达;和 用于长期保存细菌的ultraflow冷冻机 所述实验中使用的菌株和细胞系。 通过向学生传授 分子克隆和基因表达，该项目正在提高他们的 学术经验，提高他们的科学意识， 过程，并为他们的科学生涯做好更充分的准备。 的 受资助机构正在将NSF的奖励与同等金额的奖励相匹配 来自非联邦来源。

The goal of this project is to introduce current techniques in molecular genetics into the undergraduate biology curriculum. The equipment it provides is making it possible to offer a new laboratory course in Molecular Biology, as well as to improve the present Cell Biology, Microbiology, and General Biology course laboratories. Approximately 170 students per year, at all undergraduate levels, will be affected by these changes. The new equipment includes a bacteriologic incubator and water bath shaker to culture bacteria for the isolation of plasmids; a CO2 incubator and laminar-flow clean benches for student work with tissue culture; microcentrifuges for DNA/RNA purification and concentration; horizontal agarose gel units and a DNA-detection camera system to separate and analyse the products of restriction enzyme digestions; a polyacrylamide slab gel unit to analyze the products of in vitro transcription reactions; fluorescence illuminators to detect the cell- surface expression of the product of a transferred gene; and an ultraflow freezer for the long-term preservation of the bacterial strains and cell lines used in the experiments described. By acquainting students with the methodology involved in molecular cloning and gene expression, this project is enhancing their academic experience, increasing their awareness of the scientific process, and preparing them more fully for scientific careers. The grantee institution is matching the NSF award with an equal sum obtained from non-Federal sources.