Transformation Studies with Thiobacillus Ferrooxidans as a Prerequisite for Strain Improvement for Strategic Metals Recovery

虽然对金属的需求在增加， 金属矿石的可获得性正在稳步下降。 在 与此同时，有毒的富含金属的废物出现在几个工业 过程中，往往污染地下水和污染 食物链 众所周知，硫杆菌等生物 氧化亚铁酶可用于使这种废物解毒。 然而，在这方面， 我们能够充分利用微生物的价值， 废金属管理受到缺乏知识的阻碍， 对T.氧化亚铁 到目前为止， 目前还没有公开的遗传学程序， 这种有机体的转化。 高效的转型 系统将允许基因操纵 T.铁氧化物，特别是提高其金属回收率 容量 这项建议旨在发展这种 一个系统。 赋予卡那霉素抗性的质粒将是 作为一种标记来测试转化能力， T.在各种条件下的氧化亚铁。 转换系统的成功开发将 为生物体的基因工程铺平了道路， 可用于从有害物质中回收有价值的金属 工业废物。

Although the requirement for metals is increasing, the availability of metallic ore is steadily decreasing. At the same time toxic metal-rich waste occurs in several industrial processes, often polluting ground water and contaminating the food chain. It is known that organisms such as Thiobacillus ferrooxidans can be used to detoxify this waste. However, our ability to take full advantage of the value of microbial waste metal management is hampered by a lack of knowledge of the genetics and biochemistry of T. ferrooxidans. To date, there are no published procedures for the genetic transformation of this organism. An efficient transformation system will permit the genetic manipulation of T. ferrooxidans specifically to improve its metal recovery capacity. This proposal is aimed at the development of such a system. Plasmids conferring kanamycin resistance will be used as a marker to test the transforming ability of T. ferrooxidans under a variety of conditions. The successful development of a transformation system will pave the way for the genetic engineering of organisms which can be used to recover valuable metals from hazardous industrial wastes.