Heterologous Protein Expression in Neurospora

脉孢菌中的异源蛋白表达

• 批准号: 8860389
• 负责人: John Marshall Ivy
• 金额: $ 5万
• 依托单位: Hawaii Biotechnology Group Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-01-01 至 1989-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8860389&HistoricalAwards=false
• 关键词: Heterologous; Protein; Expression; Neurospora

The proposed research will investigate the ability of Neurospora crassa to express and secrete biologically active mammalian proteins. Although bacteria and yeast have been genetically engineered for this purpose, recombinant products from these organisms often require additional purification and processing. Filamentous fungi, including Neurospora can efficiently express, modify and secrete heterologous proteins in significant quantities. In the experiments outlined here, cDNAs encoding rat relaxin and bovine chymosin will be placed under the regulation of N. Crassa beta-tubulin gene promoter and terminator, and the ability to express these genes and secrete the proteins will be determined. If successful, these studies will enable the development of an alternate expression system in Neurospora crassa at a lower cost.

这项研究将调查粗糙脉孢菌 表达和分泌具有生物活性的哺乳动物蛋白。 虽然 细菌和酵母已经为此目的进行了基因工程改造， 来自这些生物体的重组产物通常需要额外的 纯化和加工。 丝状真菌，包括脉孢菌属 能够高效表达、修饰和分泌异源蛋白， 数量可观。 在这里概述的实验中，cDNA 将编码大鼠松弛素和牛凝乳酶的基因置于 调节N. Crassa β-微管蛋白基因启动子和终止子，和 表达这些基因和分泌蛋白质的能力将 测定 如果成功的话，这些研究将能够开发出一种替代药物， 以较低的成本在粗糙脉孢菌中构建表达系统。