Heterologous Protein Expression in Neurospora

这项拟议的研究将调查粗糙脉孢菌表达和分泌具有生物活性的哺乳动物蛋白的能力。尽管细菌和酵母已经为此目的进行了基因工程，但这些生物的重组产品通常需要额外的提纯和加工。丝状真菌，包括脉孢菌，可以高效表达、修饰和分泌大量的异源蛋白。在这里概述的实验中，编码大鼠松弛蛋白和牛凝乳酶的cDNA将被置于粗毛杆菌β-微管蛋白基因启动子和终止子的调控下，并将确定表达这些基因和分泌蛋白质的能力。如果成功，这些研究将能够以较低的成本在粗糙脉孢菌中开发另一种表达系统。

The proposed research will investigate the ability of Neurospora crassa to express and secrete biologically active mammalian proteins. Although bacteria and yeast have been genetically engineered for this purpose, recombinant products from these organisms often require additional purification and processing. Filamentous fungi, including Neurospora can efficiently express, modify and secrete heterologous proteins in significant quantities. In the experiments outlined here, cDNAs encoding rat relaxin and bovine chymosin will be placed under the regulation of N. Crassa beta-tubulin gene promoter and terminator, and the ability to express these genes and secrete the proteins will be determined. If successful, these studies will enable the development of an alternate expression system in Neurospora crassa at a lower cost.