Control of Respiratory Electron Flow: A Molecular Genetic Basis for the Alternative Oxidase

这项研究利用遗传学的方法， 氧化酶来阐明线粒体的调节 对整体能源和碳平衡的贡献。 最初，焦点集中在被鉴定为 编码至少部分替代氧化酶。 有 大量的生理数据， 替代氧化酶和如此少的分子/遗传数据， 这种氧化酶的一级序列应该能给出答案 目前还无法预测 的第二部分 该项目利用了来自三种不同的氧化酶基因， 模型系统以了解监管 呼吸电子流之间的细胞色素和 替代途径。% 植物、真菌、蓝细菌和许多其他生物都具有 一种是正常的细胞色素呼吸途径， “替代”，途径。 这条途径的重要性在于 与这些地区的总能量和碳平衡的关系 生物体是显而易见的，然而，我们的生物化学和遗传 关于其身份的知识极为有限。 本研究将为今后的研究提供结构基础。 替代途径的“替代氧化酶”。 它将 还阐明了一种可能的机制， 环境诱导的植物交替氧化酶能力 组织中 一个电子流调节模型， 细胞色素和呼吸的替代途径将 还将制定一个框架， 呼吸电子调节假说 流动以及通过基因操作的遗传基础， 转换，以测试这些假设。*//

This research utilizes a genetic approach for the alternative oxidase to elucidate the regulation of the mitochondrial contribution to overall energy and carbon balance. Initially, the focus centers on the gene(s) identified as encoding at least part of the alternative oxidase. There is a vast amount of physiological data concerning the alternative oxidase and so little molecular/genetic data that the primary sequence of this oxidase should yield answers that cannot be predicted at this time. The second portion of this project utilizes alternative oxidase genes from three model systems in order to understand the regulation of respiratory electron flow between the cytochrome and alternative pathways.%%% Plants, fungi, cyanobacteria and many other organisms possess both a normal cytochrome pathway of respiration and another, "alternative", pathway. The significance of this pathway in relation to total energy and carbon balance in these organisms is evident, yet, our biochemical and genetic knowledge concerning its identity is extremely limited. This research will provide a structural basis for the "alternative oxidase" of the alternative pathway. It will also elucidate a possible mechanism for the developmental and environmentally induced alternative oxidase capacity in plant tissues. A model for the regulation of electron flow between the cytochrome and alternative pathways of respiration will also be developed, providing a framework for future hypotheses concerning the regulation of respiratory electron flow as well as a genetic basis through gene manipulation and transformation, to test such hypotheses.***//