Mitochondrial Nuclear Regulation of Respiration

9723573 McIntosh植物线粒体有两个末端氧化酶，细胞色素氧化酶和交替氧化酶，以及能量流动的选择途径。 交替氧化酶是由一个小的核基因家族编码的，似乎是连接线粒体能量平衡和生物合成碳代谢，特别是在胁迫条件下。 本研究的目的是利用交替氧化酶的合成和激活来研究植物的核/线粒体通讯，并利用转基因植物来研究交替途径呼吸的碳/能量平衡功能。 这一目标将通过对植物的遗传、分子、生物化学和生理学分析来实现，其中交替氧化酶被改变，并在体外测定中。

9723573 McIntosh Plant mitochondria have two terminal oxidases, cytochrome oxidase and the alternative oxidase and a choice of pathways for energy flow. Alternative oxidase is encoded by a small nuclear gene family and appears to link mitochondrial energy balance and biosynthetic carbon metabolism, especially under stress condition. The goals of this study are to investigate nuclear/mitochondrial communication in plants using synthesis and activation of the alternative oxidase, and to investigate carbon/energy balance function of the alternative pathway respiration employing transgenic plants. The goal will be approached using genetic, molecular, biochemical and physiological analysis of plants in which the alternative oxidase is altered and in in-vitro assays.