Role of a Galactose-Binding Insect Lectin in Defense Response (s) Against Invading Pathogens

半乳糖结合昆虫凝集素在针对入侵病原体的防御反应中的作用

• 批准号: 8905060
• 负责人: Drion Boucias
• 金额: --
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-11-01 至 1991-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8905060&HistoricalAwards=false
• 关键词: Role; Galactose; Binding; Insect; Lectin

The purpose of the proposed research is further to elucidate the role of insect lectins in the defense response against pathogens. A galactose binding lectin from Spodoptera exigua (beet armyworm) will be used in these studies. This lectin is easily extracted from larval hemolymph and has been shown to opsonize potential fungal pathogens which have surface galactose residues. Monoclonal antibodies against the lectin will be prepared in order to localize the molecule in insect tissues. Light and electron microscopy will be used for these studies and in experiments using direct probes to localize lectin receptors on cell walls of selected microorganisms. Antimicrobial properties of the lectin will be assessed as well as the fate of opsonized microbes phagocytosed by host granulocytes. Viability of ingested cells will be determined by conventional plating methods and by use of a methylene blue staining assay. Finally, the distribution of lectin receptors on both immunocompetent and immunosuppressed granulocytes will be examined. Lectins are specific sugar-binding proteins that have been detected in the body fluids of numerous animal species. Although little is known about the function of these molecules, it has been suggested on the basis of a variety of sketchy evidence that in insects they may function in defense against bacteria or fungi by coating the microorganisms and promoting their removal by scavenger cells in the insect's blood. This research will examine this hypothesis more thoroughly and should elucidate the role(s) of lectins in an insect's defense against invading microorganisms.

拟议研究的目的是进一步阐明 昆虫凝集素在病原体防御反应中的作用。 甜菜夜蛾半乳糖结合凝集素 将用于这些研究。 这种凝集素很容易提取 从幼虫血淋巴中提取， 具有表面半乳糖残基的真菌病原体。 抗凝集素的单克隆抗体将在 以便将分子定位在昆虫组织中。 光和 电子显微镜将用于这些研究， 使用直接探针定位凝集素受体的实验 选定微生物的细胞壁。 抗微生物性质 的凝集素将被评估以及命运的调理 被宿主粒细胞吞噬的微生物。 可行性 摄取的细胞将通过常规的平板接种方法测定 并通过使用亚甲蓝染色测定。 最后 凝集素受体在免疫活性和 将检查免疫抑制的粒细胞。 凝集素是特异性糖结合蛋白， 在许多动物的体液中检测到。 虽然 对这些分子的功能知之甚少， 根据各种粗略的证据， 在昆虫中，它们可以起到防御细菌或真菌的作用 通过涂覆微生物并促进它们的去除， 昆虫血液中的清道夫细胞 这项研究将 更彻底地研究这个假设，并应阐明 凝集素在昆虫防御入侵中的作用 微生物的