Pathways of Pyrophosphate Utilization and Sucrose Metabolismin Higher Plants

高等植物中焦磷酸盐利用和蔗糖代谢途径

• 批准号: 8905458
• 负责人: Clanton Black
• 金额: $ 8.5万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8905458&HistoricalAwards=false
• 关键词: Pathways; Pyrophosphate; Utilization; Sucrose; Metabolismin

This research is studying the role of pyrophosphate as an alternative (to ATP) energy source and phosphate donor in higher plants. Research is concentrating on measurements of the pyrophosphate content of plant tissues and the variations that occur between tissues that serve as sources and sinks for carbohydrate metabolism. The pyrophosphate-utilizing enzyme, pyrophosphatase, will be purified and used to generate antibodies against the protein for use in cellular localization studies. The role of pyrophosphate in ethylene production is to be ascertained by correlating pyrophosphate measurements with the production of ethylene during the climacteric response in ripening banana.%%% The regulation of carbohydrate metabolism in higher plants is integral to the processes by which plants grow and develop. This research is looking at the role of a specific high energy compound, pyrophosphate, as both an energy source and regulatory molecule in higher plants. Studies will focus on attempts to purify a major enzyme associated with pyrophosphate metabolism and ultimately determine its cellular site of action. In addition work is being carried out on the contributions of pyrophosphate in the fruit ripening response in banana.***//

这项研究正在研究焦磷酸盐作为一种 替代（ATP）能源和磷酸盐供体， 高等植物 研究集中在测量 植物组织中焦磷酸盐含量及其变化 发生在作为源和汇的组织之间 碳水化合物的代谢。 焦磷酸盐利用 酶，焦磷酸酶，将被纯化并用于 产生针对蛋白质的抗体，用于细胞免疫， 本地化研究。 焦磷酸盐在乙烯中的作用 通过将焦磷酸盐 乙烯生产过程中的测量 成熟香蕉的跃变型反应。 高等植物碳水化合物代谢的调节 是植物生长发育过程中不可或缺的。 这项研究着眼于一种特定的高 能量化合物，焦磷酸盐，作为一种能量来源， 高等植物中的调节分子。 研究将集中在 试图纯化与之相关的主要酶 焦磷酸盐代谢，并最终确定其 细胞作用位点。 此外，工作正在进行中。 焦磷酸盐在水果中的作用 香蕉的成熟反应 *//