Pathways of Pyrophosphate Utilization and Sucrose Metabolismin Higher Plants

本研究旨在研究焦磷酸盐在高等植物中作为替代(ATP)能源和磷酸盐供体的作用。研究集中在测量植物组织的焦磷酸盐含量以及作为碳水化合物新陈代谢的源和汇的组织之间发生的变化。焦磷酸利用酶焦磷酸酶将被提纯，并用于产生抗体，用于细胞定位研究。焦磷酸盐在乙烯产生中的作用是通过将焦磷酸盐的测量与成熟香蕉的跃变反应中的乙烯产生相关联来确定的。高等植物碳水化合物代谢的调节是植物生长发育过程中不可或缺的一部分。这项研究着眼于一种特定的高能化合物焦磷酸盐，它既是高等植物的能源，也是调节分子。研究将集中在试图提纯一种与焦磷酸盐代谢相关的主要酶，并最终确定其细胞作用部位。此外，正在开展关于焦磷酸盐在香蕉果实成熟反应中的作用的研究。

This research is studying the role of pyrophosphate as an alternative (to ATP) energy source and phosphate donor in higher plants. Research is concentrating on measurements of the pyrophosphate content of plant tissues and the variations that occur between tissues that serve as sources and sinks for carbohydrate metabolism. The pyrophosphate-utilizing enzyme, pyrophosphatase, will be purified and used to generate antibodies against the protein for use in cellular localization studies. The role of pyrophosphate in ethylene production is to be ascertained by correlating pyrophosphate measurements with the production of ethylene during the climacteric response in ripening banana.%%% The regulation of carbohydrate metabolism in higher plants is integral to the processes by which plants grow and develop. This research is looking at the role of a specific high energy compound, pyrophosphate, as both an energy source and regulatory molecule in higher plants. Studies will focus on attempts to purify a major enzyme associated with pyrophosphate metabolism and ultimately determine its cellular site of action. In addition work is being carried out on the contributions of pyrophosphate in the fruit ripening response in banana.***//