Characterization of Metabolism and Excretion Patterns of Pulpmill Effluent Components by Fish

中长期研究：鱼类对纸浆厂废水成分的代谢和排泄模式的表征。本奖项推荐是根据外国英才中心长期和中期研究计划提出的。该计划旨在使年轻的美国科学家和工程师能够在国外被证明是优秀的研究机构进行长期研究。奖项为开展联合研究和利用外国独特或互补的设施、专门知识和实验条件提供了机会。该奖项将支持田纳西大学的玛莎·布莱克博士与芬兰若恩苏大学的艾莫·奥卡里博士进行为期12个月的研究访问。研究人员将使用实验室和现场暴露的分级方法来研究鱼类通过鳃、皮肤、肾脏和肝胆系统消除两种纸浆厂流出物成分(脱氢松酸和3，4，5-三氯愈创木酚)和苯并？a！芘的情况。鲑鱼的复合排泄模式和代谢物形成速率将使用试验室进行量化。模型化合物的排泄将在有或没有预先暴露于稀浆厂废水或&gt；-纳豆黄酮的情况下进行表征，后者是单加氧酶系统的有效诱导剂。在受控条件下进行这些实验室实验后，白鱼将被关在距离污水排放区3-15公里处的一个接收纸浆厂污水的湖泊中。在6个月的暴露期内，以指定的间隔移走鱼将被分析脱氢松酸和3，4，5-三氯愈创木酚的代谢酶活性和代谢物。实验室和实地研究的结果将被用来评估异种排泄物在鱼类适应长期低水平污染方面的作用。奖励建议提供资金，以酌情支付国际旅行、当地出国旅行、津贴、受抚养人津贴(如果适用)以及为美国本土机构提供250.00美元的固定行政津贴。

Long and Medium Term Research: Characterization of Metabolism and Excretion Patterns of Pulpmill Effluent Components by Fish. This award recommendation is made under the Program for Long and Medium-Term Research at Foreign Centers of Excellence. The program is designed to enable young U.S. scientists and engineers to conduct long-term research abroad at research institutions of proven excellence. Awards provide opportunities for the conduct of joint research and the utilization of unique or complementary facilities, expertise and experimental conditions in foreign countries. The award will support a 12 month research visit by Dr. Marsha Black, University of Tennessee, with Dr. Aimo Oikari of the University of Joensuu, Finland. The researchers will use a tiered approach using both laboratory and field exposures to study the elimination of two pulpmill effluent components (dehydroabietic acid and 3,4,5- trichloroguiacol) and benzo?a!pyrene by fish via the gills, skin, kidney, and hepatobiliary system. Compound excretion patterns and rates of metabolite formation by trout will be quantified using an experimental chamber. Excretion of the model compounds will be characterized with and without preexposure to dilute pulpmill effluent or >-napthoflavone, a potent inducer of the monooxygenase enzyme system. Following these laboratory experiments under controlled conditions, whitefish will be caged in a lake receiving pulpmill effluents at locations 3-15 km from the effluent discharge area. Fish removed at specified intervals over the 6 month exposure period will be analyzed for metabolizing enzyme activities and metabolites of dehydroabietic acid and 3,4,5-trichloroguiacol. Results from laboratory and field studies will be used to evaluate the role of xenobiotic excretion in the adaptation of fish to chronic low-level pollution. The award recommendation provides funds to cover, as appropriate, international travel, local travel abroad, stipend, dependents' allowance, if applicable, and a flat administrative allowance of $250.00 for the U.S. home institution.