Metabolic Pathway Engineering of the 1,3 - Propanediol System

1,3-丙二醇系统的代谢途径工程

• 批准号: 8910077
• 负责人: Douglas Cameron
• 金额: $ 6.67万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8910077&HistoricalAwards=false
• 关键词: Metabolic; Pathway; Engineering; Propanediol; System

Metabolic Pathway Engineering (MPE), defined as the design, analysis, and construction of biochemical pathways, is an emerging technology with broad implications for the production of chemicals and the degradation of toxic compounds. MPE involves adding genetic material to a host organism to modify an existing pathway or to create a new one. The objective of this project is to directly address key problems associated with MPE by constructing a new pathway in E. coli. The model pathway selected for this research is the conversion of glycerol to 1,3 - propanediol as carried out by Klebsiella pneumoniae. The first step is to characterize the carbon flux and intracellular intermediate concentrations of K. pneumoniae, as a basis of comparison with the engineered E. coli. The final step is to then study the function of the new pathway in E. coli and investigate its interactions with the host metabolism. This work is expected to provide new insight into MPE and to provide a well characterized model system for further research. In addition, 1,3 - propanediol is a useful chemical intermediate, and it is possible that this work will lead to improvements in its production.

代谢途径工程（MPE），定义为设计，分析， 和生物化学途径的构建，是一项新兴技术， 对化学品生产和降解的广泛影响 有毒化合物。 MPE涉及向宿主添加遗传物质 生物体修改现有的途径或创建新的途径。 的 该项目的目标是直接解决相关的关键问题 通过在E.杆菌 模式路径 本研究选择的是甘油转化为1，3- 丙二醇，如由肺炎克雷伯氏菌进行的。 第一步是 以表征碳通量和细胞内中间体 K.浓度肺炎，作为比较的基础 工程E.杆菌 最后一步是研究 E.并研究其与宿主的相互作用 新陈代谢. 这项工作有望为MPE提供新的见解， 为进一步的研究提供一个良好的表征模型系统。 在 此外，1，3-丙二醇是有用的化学中间体， 这项工作可能会导致其生产的改进。