Minimizing the Genome of Escherichia Coli

最小化大肠杆菌基因组

• 批准号: 9205918
• 负责人: Douglas Cameron
• 金额: $ 4.19万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-03-15 至 1993-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9205918&HistoricalAwards=false
• 关键词: Minimizing; Genome; Escherichia; Coli

Bacteria contain numerous genes and complex regulatory systems that are necessary for survival in nature, but are not required for growth under controlled conditions in a bioreactor. The inherent complexity of bacteria makes bioprocess development unpredictable and modeling of bacterial growth and metabolism difficult. The goal of this project is to reduce the complexity of Escherichia coli, the best understood bacterium and one of industrial importance. This will be carried out by selective deletions of the genome of the bacterium. Successful deletions will be those that allow normal growth on a defined medium.

细菌包含许多基因和复杂的调控系统，它们是在自然界中生存所必需的，但不是在生物反应器中受控条件下生长所必需的。细菌固有的复杂性使得生物过程的发展不可预测，细菌生长和新陈代谢的建模也变得困难。该项目的目标是降低大肠杆菌的复杂性，大肠杆菌是人们最了解的细菌之一，也是具有工业重要性的细菌之一。这将通过选择性地删除细菌的基因组来实现。成功的删除将是那些允许在定义的介质上正常生长的删除。