Construction and Evaluation of Self-Luminescent Biosensors

自发光生物传感器的构建与评估

• 批准号: 8915294
• 负责人: David Holmes
• 金额: $ 20万
• 依托单位: Rensselaer Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1991-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8915294&HistoricalAwards=false
• 关键词: Construction; Evaluation; Self; Luminescent; Biosensors

Biosensors will be constructed and evaluated for monitoring mercury and toluene in the environment and for monitoring the metabolic activity of cells in a bioreactor. The biosensors all operate on the same principle of using microorganisms containing the lux genes to produce light in response to induction by an analyte. The light will be collected with a simple semiconductor juction (diode). The microorganisms and the photodiode will be interfaced to produce a biosensor devise. Advantages of our approach include immediate commercial opportunities, appropriate sensitivity and selectivity for the analytes, existing sophistication of light collection and analysis, and generalizability of the concept to produce a whole range of similar devices capable of reporting other environmental toxicants, drugs, pharmaceuticals, organisms and many other biological compounds.

将构建和评估生物传感器，用于监测环境中的汞和甲苯以及监测生物反应器中细胞的代谢活动。 所有生物传感器的工作原理都相同，即使用含有 lux 基因的微生物响应分析物的诱导而产生光。 光将通过简单的半导体结（二极管）收集。微生物和光电二极管将连接起来以产生生物传感器装置。 我们方法的优点包括直接的商业机会、分析物的适当灵敏度和选择性、现有的光收集和分析的复杂性，以及生产能够报告其他环境毒物、药物、药品、生物体和许多其他生物化合物的一系列类似设备的概念的普遍性。