A Study of Isoprene Emission from Plants

树木排放的异戊二烯是碳氢化合物的重要来源 大气中的气体。 在这个项目中， 将调查橡树的异戊二烯排放量。 这将 有助于我们对大气层的理解， 预测大气层的变化。 异戊二烯由植物释放， 以光合作用速率的2%或更多的速率进入大气。 植物的这种异戊二烯排放是 生物圈和大气层;据估计， 异戊二烯每年都被释放到大气中。 PI建议 确定什么条件加速异戊二烯排放的速度， 完整的叶子，并确定什么样的影响，如果有的话，异戊二烯对 植物生理学 具体而言，他将测试（1）提议的 异戊二烯排放与光呼吸的关系; 异戊二烯排放与光反应的关系 光合作用，以及（3）异戊二烯排放与 光合碳代谢 此外，实验将 协助提出假设。 实验将 用完整的叶子进行。 异戊二烯的形成可能涉及 叶绿体内的糖酵解代谢，这也是 合成酪氨酸、色氨酸、亮氨酸、异亮氨酸和缬氨酸， 人体营养必需的氨基酸 初步研究如何 开始异戊二烯合成和糖酵解的生物化学研究 叶绿体内的反应也将进行。

Isoprene emission from trees is a significant source of hydrocarbon gases in the atmosphere. In this project the conditions which promote isoprene emission from oak trees will be investigated. This will contribute to our understanding of the atmosphere and our ability to predict changes in the atmosphere. Isoprene is released by plants to the atmosphere at a rate of 2% or more of the rate photosynthesis. This isoprene emission from plants is an important interface between the biosphere and the atmosphere; it is estimated that 350 X 1012 g of isoprene is released to the atmosphere each year. The PI proposes to determine what conditions accelerate the rate of isoprene emission from intact leaves and to determine what effects, if any, isoprene has on the physiology of plants. Specifically, he will test (1) the proposed relationship between isoprene emission and photorespiration, (2) the relationship between isoprene emission and the light reactions of photsynthesis, and (3) the relationship between isoprene emission and photosynthetic carbon metabolism. In addition experiments will be undertaken to assist in generating hypotheses. Experiments will be conducted with intact leaves. Isoprene formation is likely to involve glycolytic metabolism inside chloroplasts, which is also required for synthesis of tyrosine, tryptophan, leucine, isoleucine and valine, amino acids essential for human nutrition. Preliminary studies of how to begin biochemical studies of isoprene synthesis and glycolytic reactions inside chloroplasts will also be undertaken.