Mating-Type Limited Meiotic Mutants in Chlamydomonas

这项建议的目的是了解BALD-2基因座在减数分裂中的作用，并识别与该基因座相互作用的其他基因。通过分离突变的秃发-2等位基因的抑制子和增强子，将使用遗传学方法来检测秃发-2基因座的突变表型范围，并识别相互作用和调节的基因。分离的突变型和野生型基本体的制备将通过二维凝胶电泳法分析多肽组成的变化，并通过电子显微镜和计算机辅助成像来分析形态变化。BALD-2基因将被克隆和测序，以用于进一步的分子研究。对减数分裂的基本过程及其遗传控制的研究将对所有研究有性繁殖生物的人有用。利用这种实验有机体获得的信息可以转移到许多动植物身上。

The goal of this proposal is to understand the role of the BALD-2 locus in meiosis and to identify other genes that interact with this locus. A genetic approach will be used to examine the range of mutant phenotypes at the BALD-2 locus and to identify interacting and regulatory genes by the isolation of suppressors and enhancers of mutant bald-2 alleles. Preparations of isolated mutant and wild-type basal bodies will be analyzed by two- dimensional gel electrophoresis for changes in the polypeptide composition and by electron microscopy and computer-assisted imaging for morphological changes. The BALD-2 gene will be cloned and sequenced for use in further molecular studies. A study of the fundamental process of meiosis and its genetic control will be useful to all who study sexually reproducing organisms. The information gained using this experimental organism can be transferred to many animals and plants.