Femto/Picosecond aser Sectroscopy of Bacteriorhodopsin

细菌视紫红质的飞秒/皮秒激光透视

• 批准号: 8918673
• 负责人: George Atkinson
• 金额: $ 20.2万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-05-15 至 1993-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8918673&HistoricalAwards=false
• 关键词: Femto; Picosecond; aser; Sectroscopy; Bacteriorhodopsin

This research program is intended to elucidate the molecular structure and dynamics of the photocycling intermediates in native, and in a series of artificial bacteriorhodopsin (BR) systems. The proton pumping process which operates in BR membranes is triggered by retinal excitation and will be examined with time- resolved laser measurements of the vibration and electronic state properties of selected amino acids in the trans-membrane protein. Vibrational Raman, absorption and fluorescence spectroscopies measured with femto/pico (10-13 sec to 10-12 sec) times resolution will be used to determine the configurational and conformational changes that occur. These photocycles are related to the molecular mechanism of vision and may have relevance to the design and synthesis of new photochromic material for optical data storage and fast optical switching.

这项研究计划旨在阐明 分子结构和光循环动力学 天然的中间体，以及一系列人工合成的中间体， 细菌视紫红质（BR）系统。 质子泵 在BR膜中操作的过程由以下触发： 视网膜兴奋，并将随着时间的推移进行检查- 解决了振动的激光测量， 中所选氨基酸的电子态性质 跨膜蛋白 振动拉曼， 吸收和荧光光谱测量， 毫微微/皮科（10-13秒至10-12秒）倍的分辨率将是 用于确定构型和构象 发生的变化。 这些光循环与分子机制有关 可能与设计有关， 新型光致变色材料合成 存储和快速光交换。