Structure-Function Studies of L-3-Hydorxyacyl Coenzyme A Dehydrogenase, Fumarase and Cytoplasmic Malate Dehydrogenase

L-3-羟基酰辅酶A脱氢酶、延胡索酸酶和细胞质苹果酸脱氢酶的结构-功能研究

• 批准号: 8941746
• 负责人: Leonard Banaszak
• 金额: $ 46.27万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-15 至 1994-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8941746&HistoricalAwards=false
• 关键词: Structure; Function; Studies; Hydorxyacyl; Coenzyme

Molecular data and detail are vital factors in understanding biochemical phenomena related to enzymes. Dr. Banaszak is carrying out an X-ray crystallographic study of L-3- hydroxyacyl CoA Dehydrogenase, cytoplasmic malate dehydrogenase (sMDH) and fumarase. Although these structural studies are at different stages, the long range objectives in each case are similar. Structural investigations of the type proposed are fundamental to understanding the mechanism of catalytic activity of enzymes which carry out essential steps in a number of fundamental biochemical pathways. Dr. Gail Rhodes, of the University of South Maine, will spend part of the summer in the laboratory in order to continue model building and refinement studies of the proteins L-3- hydroxyacyl Coenzyme A dehydrogenase and cytoplasmic malate dehydrogenase. This opportunity will enable him to develop a research program related to protein structure at his University where his research interests focus on the molecular structure of proteins, protein model building, structure refinement and the comparison of protein structures.

分子数据和细节是理解 与酶有关的生物化学现象。 巴纳斯扎克博士是 对L-3进行X射线晶体学研究， 羟酰辅酶A脱氢酶，胞质苹果酸 脱氢酶（sMDH）和脱氢酶。 虽然这些结构 研究处于不同阶段， 每个案例都是相似的。 类型的结构调查 提出的是根本的理解机制， 进行基本步骤的酶的催化活性 在一些基本的生化途径中。 南缅因州大学的盖尔罗兹博士将花费 夏天的一部分时间在实验室里， 蛋白质L-3- 羟酰辅酶A脱氢酶和胞质苹果酸 脱氢酶。 这个机会将使他能够发展一个 与蛋白质结构相关的研究项目 他的研究兴趣集中在 蛋白质的分子结构，蛋白质模型的建立， 蛋白质的结构精修与比较 结构.