Presidential Young Investigator Award: Role of Nucleic Acid Conformation in Genetic Regulation

总统青年研究员奖：核酸构象在遗传调控中的作用

• 批准号: 8958280
• 负责人: Juli Feigon
• 金额: $ 27.45万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-15 至 1995-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8958280&HistoricalAwards=false
• 关键词: Presidential; Young; Investigator; Award; Role

In order to understand the role of nucleic acid conformation in genetic regulation, Dr. Feigon plans to: 1) continue studies of DNA oligomers containing potential B-Z junctions and triple stranded DNAs; 2) investigate echinomycin-DNA complexes; 3) examine how ECORI restriction enzyme and methylase bind specifically to the DNA sequence GAATTC; and 4) the study the interaction of the typtophan repressor with operator DNA. This project is concerned with the range of conformations that nucleic acids adopt, both free in solution and in complex with proteins and drugs, and in the specific factors that determine sequence specific binding of proteins and other ligands to nucleic acids. The primary research tool for this project is high field nuclear magnetic resonance spectroscopy, particulary two-dimensional, to elucidate structure and dynamics.

为了了解核酸构象在遗传调控中的作用，Feigon博士计划：1)继续研究含有潜在B-Z连接和三链DNA的DNA低聚物；2)研究棘霉素- dna复合物；3)研究ECORI限制性内切酶和甲基化酶如何特异性结合DNA序列GAATTC；4)色氨酸抑制因子与操作子DNA的相互作用研究。该项目涉及核酸在溶液中游离和与蛋白质和药物结合时所采用的构象范围，以及决定蛋白质和其他配体与核酸特异性结合的序列的特定因素。本项目的主要研究工具是高场核磁共振波谱，特别是二维，以阐明结构和动力学。