An Optical Microscope for Imaging Three-Dimensional Semitransparent Structures

该奖项提供资金，以确定构建电光显微镜的可行性，该显微镜具有产生三维半透明物体的高质量，定量图像的能力，而无需在成像前对标本进行物理切片。该显微镜通过使用衍射层析成像的滤波反向传播算法对结构的多个Gabor全息图进行数字处理，从而产生物体的三维复折射率的重建。第一阶段计划的主要目标是确定与重建过程对噪声和其他误差源的敏感性相关的潜在显微原理的实用性，以及确定这种显微镜在非理想操作条件下的成像性能。该系统的面包板原型设计将在第一阶段完成。

This award provides funds to determine the feasibility of constructing an electro-optical microscope that has the capability of yielding high quality, quantitative images of three-dimensional semitransparent objects without the need of physically slicing the specimen prior to imaging. The microscope generates a reconstruction of the three-dimensional complex index of refraction of an object by digitally processing multiple Gabor holograms of the structure using the filtered back-propagation algorithm of diffraction tomography. The Phase I program has as its principal goal the determination of the practicality of the underlying microscopic principle in relation to the sensitivity of the reconstruction procedure to noise and other error sources and the determination of the imaging performance of such a microscope under less than ideal operating conditions. A prototype breadboard design of the system will be completed during the Phase I program.