Mechanism(s) of Plant Response to Suboptimal and Adverse Irradiance

植物对次优和不利辐照度的反应机制

• 批准号: 9003928
• 负责人: Anastasios Melis
• 金额: $ 13.55万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9003928&HistoricalAwards=false
• 关键词: Mechanism; Plant; Response; Suboptimal; Adverse

Irradiance stress directly affects the structure of the photosystems in plant chloroplasts. These adjustments apparently help optimize photosynthesis and allow the plant to retain efficient growth under suboptimal and/or adverse irradiance conditions. The overall goal of this research is to understand the fundamental mechanism of plant response to suboptimal and adverse irradiance. Components of thylakoid membranes which are found in chloroplasts will be studied. Specific goals of this proposal are to identify the primary receptor of the signal (irradiance stress) and elucidate the first step in the signal transduction and transformation process leading to a plant response. Experimental methodologies and approaches to be employed include absorbance difference spectrophotometry, gel electrophoresis and immunoblot (western) analysis, radioactive labelling and autoradiography, and DNA-RNA hybridization (northern analysis). This research addresses a novel idea, namely that photosynthetic pigments act in the primary step of adverse irradiance recognition and that signal transduction is mediated by electron transport reactions in the thylakoid membrane. There is preliminary evidence to suggest that such a molecular feedback control mechanism guides plant response to irradiance stress and this proposal will examine this mechanism. Results from this research will improve our understanding of the mechanisms by which photosynthetic plants perceive and adapt to irradiance stress including ultraviolet radiation (UV-B). This will have implications for predicting plant response to potential increases in UV-B that may result from depletion of stratospheric ozone in the atmosphere.

光照胁迫直接影响植物光合系统的结构， 植物叶绿体 这些调整显然有助于优化 光合作用，并允许植物保持有效的生长下， 次优和/或不利的辐照条件。 的总目标 本研究旨在了解植物生长的基本机制， 对次优和不利辐照的反应。 类囊体组分 将研究在叶绿体中发现的膜。 具体 该提案的目标是确定主要受体的 信号（辐照强制降解）并阐明信号中的第一步 转导和转化过程导致植物反应。 拟采用的实验方法和途径包括 吸光度差分光光度法、凝胶电泳和 免疫印迹（western）分析、放射性标记和 放射自显影和DNA-RNA杂交（北方分析）。 这项研究提出了一个新的想法，即光合作用 色素在识别不利辐照度的主要步骤中起作用， 信号转导是由电子传递反应介导的， 类囊体膜 有初步证据表明， 这种分子反馈控制机制引导植物响应， 辐射压力和这个建议将研究这一机制。 这项研究的结果将提高我们对 光合作用植物感知和适应 辐照胁迫包括紫外线辐射（UV-B）。 这将 对预测植物对潜在增加的反应有影响 在紫外线-B，可能造成的平流层臭氧消耗， 气氛