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Mechanism and Regulation of Ethylene Biosynthesis

乙烯生物合成机制及调控

基本信息

批准号：
9004129
负责人：
Shang Fa Yang
金额：
$ 33.6万
依托单位：
University of California-Davis
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1990
资助国家：
美国
起止时间：
1990-07-15 至 1993-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9004129&HistoricalAwards=false
关键词：
Mechanism Regulation Ethylene Biosynthesis

项目摘要

This award provides further funding for Dr. S. F. Yang, to enable him to continue his research on ethylene biosynthesis. Assisted by other NSF-supported younger collaborators, Yang has demonstrated that ethylene is synthesized from methionine via S- adenosylmethionine and 1-aminocyclopropane-1-carboxylic acid. The principal objectives of the project now to be carried out by Dr. Yang are (a) to purify fully and characterize 1- aminocyclopropane-1-carboxylic acid synthase and to determine the amino acid sequence around the active site of this enzyme isolated from apples and other plant tissues, (b) to clone the gene for this enzyme from apples, (c) to characterize the inactivation of the synthase by S-adenosylmethionine in relation to the enzyme's in vivo turnover, and (d) to characterize enzymes involved in the so-called methionine cycle. Ethylene is a plant hormone responsible for the control of numerous aspects of plant growth and development. This hormone is commercially important in agriculture and in relation to post- harvest crop physiology. The work to be pursued here is aimed at obtaining basic knowledge that can later be used as a basis for manipulating the levels of ethylene in plants, either upward or downward. This is a promising approach to optimizing certain agricultural systems. Dr. Yang's studies will provide a useful theoretical basis from which ethylene production by crop plants can be regulated via biotechnology.

该奖项为杨少芳博士提供了进一步的资助，使他能够继续他在乙烯生物合成方面的研究。在其他nsf资助的年轻合作者的协助下，Yang证明了通过S-腺苷甲硫氨酸和1-氨基环丙烷-1-羧酸由蛋氨酸合成乙烯。杨博士目前正在进行的项目的主要目标是(a)完全纯化和表征1-氨基环丙烷-1-羧酸合成酶，并确定从苹果和其他植物组织中分离的该酶活性位点周围的氨基酸序列，(b)从苹果中克隆该酶的基因。(c)表征s -腺苷蛋氨酸对合成酶的失活与酶的体内周转的关系，以及(d)表征参与所谓蛋氨酸循环的酶。乙烯是一种植物激素，负责控制植物生长发育的许多方面。这种激素在农业和收获后作物生理方面具有重要的商业意义。这里进行的工作的目的是获得基本知识，这些知识以后可以用作操纵植物中乙烯水平的基础，无论是向上还是向下。这是优化某些农业系统的一种很有前途的方法。杨博士的研究将为通过生物技术调节作物的乙烯产量提供有用的理论基础。