Molecular Analysis of the ETR Locus: a Gene Involved in Ethylene Responses in Arabidopsis thaliana

ETR 基因座的分子分析：拟南芥乙烯反应相关基因

• 批准号: 9005164
• 负责人: Anthony Bleecker
• 金额: $ 28.5万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9005164&HistoricalAwards=false
• 关键词: Molecular; Analysis; ETR; Locus; Gene

The plant hormone, ethylene, regulates a wide range of physiological and developmental processes in higher plants. While a significant amount of progress has recently been made in the elucidation of the ethylene biosynthetic pathway and in the molecular bases for a number of ethylene regulated processes, virtually nothing is known about the receptor and associated signal transduction pathways in plants. The goal of this proposal is the molecular cloning and characterization of the ETR locus in Arabidopsis thalina. Mutations in the ETR locus have been isolated which cause insensitivity to a number of ethylene responses in the plant. Physiological evidence indicates that the mutations directly effect the ethylene receptor. From a chromosome walk, an 18 kb region of cloned genomic DNA has been obtained which appears to carry the ETR gene. The investigators intend to identify the specify sequences in this region that represent the ETR gene and to obtain full length cDNA clones of the mutant and wild type genes. The cDNA clones will also be used to manipulate the level of expression of the wild type and mutant gene products in transgenic Arabidopsis and in a number of other plant species. Engineered mutations in the gene will allow an exploration of the dominant nature of the etr phenotype.

植物激素乙烯在高等植物中调控着广泛的生理和发育过程。虽然最近在乙烯生物合成途径和乙烯调控过程的分子基础的阐明方面取得了重大进展，但实际上对植物中的受体和相关的信号转导途径一无所知。本研究的目的是对拟南芥ETR基因座进行分子克隆和鉴定。ETR基因座的突变已经被分离出来，导致植物对许多乙烯反应不敏感。生理证据表明，突变直接影响乙烯受体。从染色体行走中，获得了一个18 kb的克隆基因组DNA区域，该区域似乎携带ETR基因。研究人员打算在该区域鉴定代表ETR基因的特定序列，并获得突变型和野生型基因的全长cDNA克隆。cDNA克隆也将用于操纵野生型和突变型基因产物在转基因拟南芥和许多其他植物物种中的表达水平。基因中的工程突变将允许对etr表型的显性性质进行探索。