Structure and Dynamics of Ribonucleic Acids

核糖核酸的结构和动力学

• 批准号: 9005767
• 负责人: Stephen Harvey
• 金额: $ 29.07万
• 依托单位: University of Alabama at Birmingham
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1994-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9005767&HistoricalAwards=false
• 关键词: Structure; Dynamics; Ribonucleic; Acids

The ribosome is a small particle composed of molecules of proteins and ribonucleic acid which is responsible for the synthesis of proteins in the cell. A wide variety of experimental methods are providing information on the structure of this particle. Dr. Harvey is developing computer modeling methods for integrating that information into models for the ribosome and for other ribonucleoprotein complexes. The procedures are capable of producing low or high resolution models, depending on the available data. The lowest resolution "succinct" models identify only the locations of the principal substructures, such as individual proteins and RNA stems, but no more detailed information; at the other extreme are all-atom models. Models of mixed resolution are also produced, with succinct models for most of the structure and all-atom detail only where sufficient information exists. These procedures produce a range of models, all consistent with the experimental data and provide quantitative statements about the uncertainties in various parts of the structure, which should be useful in guiding future experimental studies. The study will involve extensions and improvements to the methodology, including more powerful algorithms for energy minimization, Monte Carlo, and molecular dynamics simulations on all-atom models. It also describes applications to the E. coli ribosome, eukaryotic small nuclear ribonucleoproteins, RNase P, and the Tetrahymena ribozyme.

核糖体是由蛋白质分子和核糖核酸组成的小颗粒，负责细胞内蛋白质的合成。 各种各样的实验方法正在提供有关该颗粒结构的信息。 哈维博士正在开发计算机建模方法，以将这些信息整合到核糖体和其他核糖核蛋白复合物的模型中。 该程序能够根据可用数据生成低分辨率或高分辨率模型。 最低分辨率的“简洁”模型仅识别主要子结构的位置，例如单个蛋白质和 RNA 茎，但没有更详细的信息；另一个极端是全原子模型。 还产生了混合分辨率模型，仅在存在足够信息的情况下才为大多数结构和全原子细节提供简洁模型。 这些程序产生了一系列模型，所有模型都与实验数据一致，并提供了结构各个部分的不确定性的定量陈述，这对于指导未来的实验研究应该很有用。 该研究将涉及方法的扩展和改进，包括更强大的能量最小化算法、蒙特卡罗和全原子模型的分子动力学模拟。 它还描述了在大肠杆菌核糖体、真核小核核糖核蛋白、RNase P 和四膜虫核酶中的应用。