Minority Experience in Biotechnology: A Core Research Facility

在过去的十年中，分子生物学的发展， 特别是重组DNA和分子克隆 技术，已经产生了巨大的和广泛的影响， 在生物学研究的许多领域。 的几名成员 CSULA的教师一直在使用这些强大的 获得重要的生物学新见解的方法 生物过程的结构和调节。 活性 探索神经活性调节的研究项目 肽的产生和释放，肌动蛋白的表达 基因在发育过程中，膜的调节 芽孢杆菌孢子形成周期中的发育 枯草杆菌，病毒蛋白基因的结构定义 在突变仙台病毒，以建立 生物学特性的分子基础，定义 分子信号引导 病毒在真核细胞中的出芽，亚细胞研究 高等植物的组织、单亲机制 线粒体和叶绿体DNA遗传 衣原体感染，以及对衣原体感染的调节机制的研究 磷饥饿诱导基因的表达 在高等植物中的表达都严重依赖于 基础设施的可用性- 在加州大学洛杉矶分校的重组DNA研究设施 的 目前的设施，主要是由我们目前获得的资助 RIMI赠款，位于这所城市大学， 大量和多样化的少数民族入学，正在提供一个 卓越的研究中心，促进培训， 少数民族学生，并将成为相关工作的重点， 邻近的许多州立大学校区。 这 应用程序请求继续支持此多用户 研究设施，特别是扩大我们的能力 开展多种类型的组织培养， 上述研究项目。

During the past decade advances in molecular biology, particularly recombinant DNA and molecular cloning technologies, have had dramatic and wide-reaching effects on many fields of biological research. Several members of the faculty at CSULA have been using these powerful methods to gain important new insights into biological structures and regulation of biological processes. Active research programs exploring the regulation of neuroactive peptide production and release, the expression of actin genes during development, the regulation of membrane development during the sporulation cycle of Bacillus subtilis, the structural definition of viral protein genes in mutant Sendai viruses in order to establish the molecular basis of their biological properties, defining the molecular signals for directing the orientation of viral budding in eukaryotic cells, studies of sub-cellular organization in higher plants, mechanisms of uniparental inheritance of mitochondrial and chloroplast DNA in Chlamydomonas, and studies of mechanisms regulating the expression of phosphate starvation-induction of gene expression in higher plants all depend heavily on the availability of the fundamental infrastructure of a well- equipped recombinant DNA research facility at CSULA. The current facility, largely funded by our currently awarded RIMI grant, located at this urban university with its large and diverse minority enrollment, is providing a center of research excellence that promotes training for minority students and will be a focus for related work at the many neighboring State University Campuses. This application requests continued support for this multi-user research facility, particularly for expanding our capacity to carry out diverse types of tissue culture, and for the individual research programs described above.