Structure and Function of the Plant Vacuolar H+-ATPase

植物液泡H-ATP酶的结构和功能

• 批准号: 9006402
• 负责人: Heven Sze
• 金额: $ 22.9万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9006402&HistoricalAwards=false
• 关键词: Structure; Function; Plant; Vacuolar; H+

The purpose of this project is to characterize the structure and function of the plant vacuolar H+-ATPase. The specific goals of the project are (i) to determine the complete subunit composition by further purification and immunoprecipitation; (ii) to study the subunit organization by dissociating and identifying the peripheral subunits; (iii) to deduce the primary structure and later to define the functional domains of selected subunits by cloning and sequencing the gene encoding the 16 kDa proteolipid; (iv) to test the function of the 16 kDa proteolipid by reconstitution and molecular genetic methods; (v) to localize the subcellular distribution of the ATPase; and (vi) to begin preliminary studies on the biosynthesis of selected ATPase subunits. Plant cells contain a large central vacuole that occupies up to 80% of the cell volume. They are important for maintaining cell turgor and for the storage of ions and metabolites. Transport processes across the vacuolar membrane (tonoplast) are controlled by electrochemical gradients that are determined in part by the tonoplast localized vacuolar ATPase (V-ATPases) which is a proton-pumping ATPase (H+-ATPase). The V-ATPases represent a class of ATPase that is present in the endomembrane system of all eukaryotic cells. Thus, investigations on the structure and function of the plant vacuolar ATPase has relevance to the function of the endomembrane system in all higher organisms.

该项目的目的是表征结构和 植物液泡H ~+-ATPase的功能。 的具体目标 该项目是（i）确定完整的亚基组成 通过进一步纯化和免疫沉淀;（ii）研究 通过分离和识别亚单位组织， （iii）推断一级结构， 随后通过以下步骤来定义所选亚基的功能结构域： 对编码16 kDa蛋白脂质的基因进行克隆和测序; (iv)为了测试16 kDa蛋白脂质的功能， 重组和分子遗传学方法;（v）定位 ATP酶的亚细胞分布;和（vi）开始 几种ATP酶生物合成的初步研究 亚单位。 植物细胞含有一个大的中央液泡， 细胞体积的80%。 它们对于维持细胞 膨压和储存离子和代谢物。 运输 跨液泡膜（液泡膜）的过程受到控制 通过电化学梯度，其部分地由 液泡膜定位的液泡ATP酶（V-ATP酶）是一种 质子泵ATP酶（H+-ATP酶）。 V-ATP酶代表一种 存在于所有动物的内膜系统中的一类ATP酶 真核细胞 因此，对结构和 植物液泡ATP酶的功能与植物的生理功能有关， 所有高等生物的内膜系统的功能。