Action of Hormones in Plant Development: Hormonal Regulationof Gene Expression in Barley Aleurone Layers

赤霉酸（GA）是一种植物激素，调节几种重要的生物活性。 生理和发育过程如种子萌发， 萌发后生长、茎伸长和花诱导。 已知GA与另一种激素脱落酸（阿坝）一起， 调节几种酶的合成和分泌， 负责利用种子中储存的营养物质。 的 酶是许多谷物发芽后生长所必需的 晶粒 这些酶中最丰富的是α-淀粉酶， 降解淀粉。 作为长期努力的一部分， GA和阿坝的作用，这项研究将集中在基因表达， 发芽的大麦种子的糊粉层。 以前的研究表明，蛋白质介导的调节 大麦-糊粉层系统中酶的表达主要在 基因转录。 该研究将调查具体的 特异性蛋白质因子与调控区的相互作用 大麦-糊粉层系统中淀粉酶调控基因的启动子。 本研究的目的包括：（1）特异性DNA的测定 与蛋白质相互作用的α-淀粉酶启动子中的序列 因素，（2）激素治疗的影响， 活性的蛋白质因子，（3）确定的重要性 糊粉层细胞内的启动子序列和（4）调查 这些因子在具有改变的遗传突变体中的活性 对GA和阿坝敏感。 这项研究的结果应该会有所改善。 我们对GA和阿坝的主要作用的理解，从而揭示了 调节激素分泌的信号转导途径的新见解 植物基因表达的调控。

Gibberellic acid (GA), a plant hormone, regulates several important physiological and developmental processes such as seed germination, post-germination growth, stem elongation and flower induction. Together with another hormone, abscisic acid (ABA), GA is known to regulate the synthesis and secretion of several enzymes which are responsible for the utilization of nutrients stored in the seed. The enzymes are essential for post-germination growth of many cereal grains. The most abundant of these enzymes is alpha-amylase, which degrades starch. As part of a long term effort to elucidate the mode of action of GA and ABA this research will focus on gene expression in the aleurone layers of germinating barley seeds. Previous research has revealed that hormone-mediated regulation of enzyme expression in the barley-aleurone system is mainly at the level of gene transcription. The proposed research will investigate specific interactions between specific protein factors with regulatory regions (promoters) of hormone-regulated genes in the barley-aleurone system. Goal for this research include: (1) determination of specific DNA sequences in the alpha-amylase promoters that interact with protein factors, (2) investigation of the effect of hormonal treatments on the activity of the protein factors, (3) determination of the importance of promoter sequences inside the aleurone cells and (4) investigation of the activity of these factors in genetic mutants with altered sensitivities to GA and ABA. Results from this research should improve our understanding of the primary action of GA and ABA, thus revealing new insights into the signal transduction pathway governing hormonal regulation of gene expression in plants.