Hormonal Regulation of Gene Expression in Cereal Aleurone Layers

谷物糊粉层基因表达的激素调节

• 批准号: 9408900
• 负责人: Tuan-hua Ho
• 金额: $ 29.2万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-01 至 1998-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9408900&HistoricalAwards=false
• 关键词: Hormonal; Regulation; Gene; Expression; Cereal

9408900 Ho The plant hormone, gibberellin (GA), exerts regulatory roles in several important physiological and developmental processes such as seed germination and post-germination growth, stem elongation and flower induction. Gibberellin and another hormone, abscisic acid (ABA), are known to regulate the synthesis and secretion of several enzymes which are responsible for the utilization of nutrients stored in cereal grains. The most abundant among these enzymes are the starch-degrading alpha-amylases and the storage protein- degrading proteinases. As the continuation of long term effort in elucidating the mode of action of these two plant hormones, the effect of hormones on the coordinated expression of alpha-amylase and protease genes will be studied in the aleurone layers of germinating barley seeds. Specifically it is planned to: 1) analyze the cis-acting elements in the protease promoter that are responsible for hormonal regulation, 2) investigate how GA and ABA regulate the level/activity of this DNA binding protein, and 3) isolate and characterize cDNA clones for "early" GA-induced genes whose expression has been suggested to be important for later alpha-amylase and proteinase expression. It is hoped that the information obtained in this proposed project will contribute to our understanding of the molecular action of these hormones on gene regulation in plants. ***

9408900 Ho 植物激素赤霉素 (GA) 在一些重要的生理和发育过程中发挥调节作用，例如种子发芽和发芽后生长、茎伸长和花诱导。 众所周知，赤霉素和另一种激素脱落酸 (ABA) 可以调节多种酶的合成和分泌，这些酶负责利用谷物中储存的营养物质。 这些酶中最丰富的是淀粉降解α-淀粉酶和储存蛋白降解蛋白酶。 随着阐明这两种植物激素作用方式的长期努力的继续，我们将在发芽大麦种子的糊粉层中研究激素对α-淀粉酶和蛋白酶基因协调表达的影响。 具体来说，计划：1) 分析蛋白酶启动子中负责激素调节的顺式作用元件，2) 研究 GA 和 ABA 如何调节该 DNA 结合蛋白的水平/活性，以及​​ 3) 分离和表征“早期”GA 诱导基因的 cDNA 克隆，这些基因的表达被认为对后来的 α-淀粉酶和蛋白酶表达很重要。 希望在这个拟议项目中获得的信息将有助于我们了解这些激素对植物基因调控的分子作用。 ***