Molecular Genetic Studies of Embryo Maturation in Maize

玉米胚胎成熟的分子遗传学研究

• 批准号: 9007481
• 负责人: Carol Rivin
• 金额: $ 26.5万
• 依托单位: Oregon State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9007481&HistoricalAwards=false
• 关键词: Molecular; Genetic; Studies; Embryo; Maturation

Developing plant embryos have the capacity to germinate or to mature. Dr. Rivin is investigating how embryo abscisic acid level, hormone sensitivity and changing water relations modulate the expression of these two developmental potentials. Her goals are to define, at the molecular level, the developmental pathways that are modulated by these physiological cues during maize embryo maturation, and to identify the genetic mechanisms that regulate their expression. To address this problem she will follow a three part program that involves both molecular and genetic approaches, focussing on mutants that fail to develop successfully through maturation phase. The specific objectives are the following: 1) Identify cDNA clones of mRNAs that are specific to embryo maturation, using her previous observations about the synthesis of maturation phase polypeptides in wild type and viviparous mutants as a basis for selection of co-regulated message sets. 2) Measure the expression of maturation mRNAs during normal maize embryogenesis and examine how this program is altered when development is disrupted genetically in viviparous and defective kernel mutants, or by manipulation of physiological factors in tissue culture. 3) Search for new transposon tagged embryo lethal mutants to collect additional loci for genetic dissection of embryo maturation, and to provide a means of cloning genes essential to embryo development. %%% In flowering plants the developing seed creates a morphologically complete embryo which goes on eventually to germinate. Embryo development takes place in two stages, the second of which is termed maturation. This project is aimed at an understanding of the factors that control this maturation process.

发育中的植物胚具有发芽或 成熟瑞文博士正在研究胚胎脱落酸 水平，激素敏感性和改变水的关系调节 这两种发展潜能的表达。她的目标 是在分子水平上定义 在玉米生长过程中， 胚胎成熟，并确定遗传机制， 调节它们的表达。为了解决这个问题，她将 遵循一个由三部分组成的程序，涉及分子和 遗传学方法，专注于未能发展的突变体 顺利度过成熟期。的具体目标 1）鉴定mRNA的cDNA克隆， 利用她以前的观察， 关于野生型中成熟期多肽的合成 和胎生突变体作为选择共调节的基础， 消息集。2)测量成熟mRNA的表达 在正常的玉米胚胎发生过程中， 当胎生动物的发育受到遗传干扰时， 和有缺陷的籽粒突变体，或通过操纵生理 组织培养中的因素。3)搜索新的转座子标记 胚胎致死突变体收集额外的基因座遗传 胚胎成熟的解剖，并提供一种方法， 克隆胚胎发育所必需的基因。 %%% 在开花植物中，发育中的种子在形态上创造了一个 最终继续发芽的完整胚。胚胎 发展分为两个阶段，第二个阶段是 称为成熟。该项目旨在了解 控制这一成熟过程的因素。