Long & Medium Term Research: Cyclin Destruction in Meiotic and Early Mitotic Cell Cycles of Xenopus Laevis

长的

• 批准号: 9007777
• 负责人: Jolinda Traugh
• 金额: $ 0.48万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-15 至 1991-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9007777&HistoricalAwards=false
• 关键词: Long; & Medium; Term; Research

Long & Medium-Term Research: Cyclin Destruction in Meiotic and Early Mitotic Cell Cycles of Xenopus Laevis This award recommendation is made under the Program for Long & Medium-Term Research at Foreign Centers of Excellence. The program seeks to enable U.S. scientists and engineers to conduct long-term research abroad at research institutions of proven excellence. Awards provide opportunities for the conduct of joint research, and the use of unique or compli- mentary facilities, expertise and experimental conditions in foreign countries. This award for a proposal sponsored by Dr. Jolinda A. Traugh, will support a 12-month postdoctoral research visit by Dr. Elizabeth Mandley to the United Kingdom to work with Dr. Michael Whitaker of the Department of Physiology of the University of the City of London, on "Cyclin Destruction in Meiotic and Early Mitotic Cell Cycles of Sea Urchins (Xenopus)." The investigators propose to investigate the mechanism of cyclin destruction during the M-phase of the rapid cell cycles of early sea urchin development. Cyclin is only unstable during M-phase, and preliminary evidence implicates a regulatory cascade of protein phosphorylation and desphosphorylation in the process. Some evidence suggests calcium may be involved in the destruction process. Cyclin destruction can be triggered in a cell-free system prepared from Xenopus eggs by addition of active cyclin/p34-cdc2 complex. Destruction requires ATP and the participation of both soluble and particulate material. This suggests that the high molecular weight multifunctional protease and hence ubiquitin may be involved. The investigators will investigate whether cylcin degradation involves ubiquitin by searching for ubiquitinated intermediates of cyclin, and by fractiona- tion. They will also investigate the role that calcium plays in the destruction process. More generally, they will attempt to purify and identify as far as possible the components of the destruction system. The award recommendation provides funds to cover, as appropriate, international travel, local travel abroad, stipend, dependents' allowance if applicable, and a flat administrative allowance of $250 for the U.S. home institution.

长期中期研究：细胞周期蛋白在减数分裂和 非洲爪蟾早期有丝分裂细胞周期 该奖项是根据长期计划提出的。 &在国外卓越中心的中期研究。 的 该计划旨在使美国科学家和工程师能够 在国外的研究机构进行长期研究， 证明卓越。 奖项提供了机会， 开展联合研究，以及使用独特或复杂的 实验设施、专业知识和实验条件 国外 这个奖项是由Jolinda A.特劳， 将支持博士为期12个月的博士后研究访问。 伊丽莎白·曼德利女士前往英国与博士合作。 迈克尔·惠特克（Michael Whitaker），纽约大学生理学系 伦敦市大学，关于“细胞周期蛋白在 海胆的减数分裂和早期有丝分裂细胞周期 （非洲爪蟾）。“调查人员建议调查 细胞周期蛋白在M期破坏的机制 海胆早期发育的快速细胞周期。 细胞周期蛋白是 仅在M期不稳定，初步证据表明 暗示了蛋白质磷酸化的调节级联， 去磷酸化的过程。 一些证据表明 钙可能参与破坏过程。 Cyclin 破坏可以在无细胞系统中触发， 通过添加活性细胞周期蛋白/p34-cdc 2从非洲爪蟾卵中分离 复杂. 销毁需要ATP和参与 可溶性和颗粒状物质。 这表明 高分子量多功能蛋白酶， 泛素可能参与其中。 调查人员将调查 通过搜索细胞周期蛋白降解是否涉及泛素， 对于细胞周期蛋白的泛素化中间体， 是的。 他们还将调查钙在 在销毁过程中。 一般来说，他们会 尽可能地净化和识别 销毁系统的组成部分。 授标建议提供资金， 适当的，国际旅行，出国当地旅行， 津贴，家属津贴（如适用）和公寓 美国本土机构的行政津贴为250美元。