Cryopreparation and Subcellular Localization of Diffusible Substances in Plant Tissues

植物组织中可扩散物质的冷冻制备和亚细胞定位

• 批准号: 9009467
• 负责人: Karen Klomparens
• 金额: $ 4.98万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1991-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9009467&HistoricalAwards=false
• 关键词: Cryopreparation; Subcellular; Localization; Diffusible; Substances

Knowledge of the localization of subcellular, diffusible substances at the electron microscope level is very important for understanding of cellular physiology. The technical approaches required to obtain this information, have thus far been applied chiefly to animal cell systems. The lack of such studies of plant tissues is due primarily to difficulties in specimen preparation related to the presence in plant cells and tissues of features such as cell walls, intercellular air spaces, and large vacuoles. For microanalysis it is critical that subcellular elements of interest not be altered in any way (addition, subtraction, movement, masking) by the specimen preparation procedures. Thus, a substantial investment of time and effort is required to develop the necessary technology for application to plant systems. The proposed research will adapt existing animal cell methods and, as necessary, develop additional techniques for the cryofixation, ultrathin sectioning, and analysis of frozen- hydrated plant specimens. If successful, the development of these techniques will permit analysis of elemental composition and localization of elements of physiological importance in plant cells. This fundamental information will be of great importance to the understanding of plant cell biology.

了解亚细胞、扩散性 电子显微镜水平的物质对于 了解细胞生理学。 的技术途径 获得这些信息所需的信息，迄今已被应用 主要是动物细胞系统。 缺乏这样的研究， 植物组织主要是由于标本的困难 与植物细胞和组织中存在的 细胞壁、胞间气隙和大的 空泡 对于微量分析，亚细胞分析是至关重要的。 不以任何方式改变感兴趣的元素（另外， 减影、移动、掩蔽） 程序. 因此，需要投入大量的时间和精力， 需要开发必要的技术应用于 植物系统 这项研究将使现有的动物 细胞方法，并在必要时开发其他技术， 冷冻固定，切片和冷冻分析- 含水植物标本 如果成功，这些技术的发展将允许 元素组成分析和元素定位 在植物细胞中的重要性。 这一根本 信息将是非常重要的了解 植物细胞生物学